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Updated: May 31, 2025

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
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CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

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通过CRISPR/Cas9n介导的编辑系统,优化Streptomyces fradiae的基因组编辑效率

Yuhan Wu1, Hui Jin1, Qiang Yu1

  • 1State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, Wuhan, China.

Applied and environmental microbiology
|January 22, 2025
PubMed
概括

这项研究开发了Streptomyces fradiae的高效CRISPR/Cas9n基因组编辑系统,克服了基因修饰方面的挑战,并改善了neomycin的生产. 新系统促进了快速的基因删除和整合,为高性能工程菌株铺平了道路.

关键词:
这就是CRISPR/Cas9D10A.这种菌种名为Streptomyces fradiae.代谢工程是代谢工程.这是一种neomycin.修复单链断裂 修复单链断裂

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