细胞间mRNA转移改变了人类多能干细胞状态.
Yosuke Yoneyama1,2, Ran-Ran Zhang3,4,5, Mari Maezawa1
1Human Biology Research Unit, Institute of Integrated Research, Institute of Science Tokyo, Bunkyo-ku, Tokyo 113-8510, Japan.
概括
跨物种信使RNA (mRNA) 转移重新编程人类干细胞. 通过直接接触,来自小鼠的mRNA促进了人类细胞的适应和纯粹状态.
科学领域:
- 细胞生物学 细胞生物学
- 干细胞生物学 干细胞生物学
- 分子生物学分子生物学
背景情况:
- 细胞间信使RNA (mRNA) 转移是哺乳动物细胞中已知的现象.
- 人类原始多能干细胞 (hPSCs) 通常在特定条件下培养.
- 了解细胞-细胞通信机制对于干细胞研究至关重要.
研究的目的:
- 研究小鼠胚胎干细胞 (mESC) 和人类原始多能干细胞 (hPSC) 之间的细胞间mRNA转移.
- 确定这种转移是否可以诱导hPSC中的重编程和适应.
- 为了确定参与这种重编程过程的特定的小鼠衍生mRNAs.
主要方法:
- 在不适合单独使用hPSC的条件下,hPSC与mESC的共同培养.
- 在hPSC中分析小鼠衍生的mRNA含量,使用转移特异性mRNA分析.
- 表面标记分析和全球基因分析,以评估hPSC状态.
- 淘汰实验针对特定的来自小鼠的转录因子mRNAs.
主要成果:
- 直接细胞接触介导的共同培养使得小鼠mRNA转移到hPSCs.
- 转移的mRNA被丰富了与转录,翻译和应激反应相关的途径.
- 在共同培养后,hPSCs获得了类似于原始的状态,由基因表达和表面标记物证实.
- 鼠标Tfcp2l1,Tfap2c和Klf4的淘汰阻止了类似于天真的转换.
结论:
- 跨物种mRNA转移可以触发哺乳动物细胞中的细胞重编程.
- 插曲性mRNA转移在细胞内和物种间的细胞通信中起作用.
- 这一发现为mRNA在细胞适应中的移动性和功能提供了新的视角.
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