使用1,9-二甲基甲蓝-DMMB和激光光的硫酸盐降解细菌的光灭活
Gustavo Vital Dos Santos1, Hesrom Fernandes Serra Moura1, Pedro Jorge Louro Crugeira2
1Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil.
Journal of photochemistry and photobiology. B, Biology
|January 22, 2025
概括
使用1,9-二甲基甲蓝 (DMMB) 的光无活化 (PI) 有效地减少了石油和天然气操作中的硫酸盐减少细菌 (SRB). 这种对环境安全的方法显示出作为传统生物杀菌剂的替代品的希望.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 硫酸盐降解细菌 (SRB) 由于设备损坏和产品退化,导致石油和天然气行业每年遭受重大经济损失.
- SRB有助于微生物学影响的腐蚀 (MIC) 和水库酸化,影响运营效率和安全.
研究的目的:
- 评估使用1,9-二甲基甲蓝 (DMMB) 作为光敏感剂 (PS) 控制SRB的光无活化 (PI) 的有效性.
- 为了确定DMMB和激光照射的最佳度,以有效无效化SRB.
主要方法:
- 在修改后的Postgate C介质中培养的SRB联盟.
- 细胞量化通过光谱测量 (λ600 nm) 在无氧,黑暗的环境中.
- 使用双向ANOVA进行统计分析,以评估DMMB度和光照对PI疗效的综合影响.
主要成果:
- 在1.5μg/ml以上的DMMB度下,抗微生物抑制率超过50% (p<0.05).
- 试验剂的疗效显著依赖于DMMB度和光密度.
- 通过1.5μg/mL的DMMB和21.6 J/cm2的光强度实现了70.58% (平均66.71%) 的减光.
结论:
- 用DMMB进行光无活化为石油和天然气行业中用于SRB控制的传统生物制剂提供了一个可行的,环境安全的替代方案.
- PI方法提供了诸如易于应用,预防细菌耐药性和与现有SRB管理策略的兼容性等优势.
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