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加巴丁通过视网膜亡和甲状腺干扰损害斑马鱼的视觉发育
Yide He1, Xiang Li1, Yan Yang1
1School of Environmental Science and Engineering, Nanjing Tech University, Jiangsu 211816, China; Sino-Portuguese Joint International Laboratory of Aquatic Toxicology, Nanjing Tech University, 30 South Puzhu Road, Nanjing, Jiangsu Province 211816, China.
Journal of hazardous materials
|January 22, 2025
概括
在水生环境中, gabapentin (GBP) 增加了斑马鱼的光敏感性,并通过引起视网膜细胞死亡和甲状腺激素干扰而损害视力. 这凸显了制药污染带来的生态风险.
科学领域:
- 环境毒理学环境毒理学
- 水生物质毒理学研究
- 发展性毒理学 发展性毒理学
背景情况:
- gabapentin (GBP) 是一种广泛用于治疗和神经病痛的药物.
- 在全球水生环境中,GBP被检测为污染物.
- 有关制药污染物对水生生物的生态影响存在担忧.
研究的目的:
- 研究环保相关度的加巴丁 (GBP) 对斑马鱼 (Danio rerio) 视觉发育的影响.
- 阐明GBP诱导的视力障碍背后的机制,包括视网膜亡和甲状腺激素失调.
主要方法:
- 斑马鱼 (Danio rerio) 暴露于 GBP (0,1,10,1000μg/L) 的环境相关度.
- 进行了行为测试 (运动活动),亡测试,光动力反应 (OKR) 测试,视网膜组织病理学,甲状腺激素测试和基因表达分析.
- 分析了参与亡,甲状腺激素调节和视网膜发育的关键基因.
主要成果:
- GBP暴露增加了斑马鱼的光敏感性和运动运动活动.
- 观察到剂量依赖的视网膜细胞死亡和增加质细胞层厚度.
- 用OKR测量的视力敏度在所有颜色刺激中显著下降.
- 甲状腺激素水平 (T3和T4) 显著降低,改变T3/T4比率.
- 基因表达分析揭示了亡,甲状腺和视网膜发育途径的失调.
结论:
- 加巴丁 (GBP) 通过视网膜亡和甲状腺激素失调扰乱斑马鱼的视觉发育.
- 即使是低度的GBP也会损害水生生物的视觉功能.
- 这些发现强调了制药污染物在水生环境中带来的生态风险,以及需要进一步进行风险评估.
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