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基于标记为单克隆抗体的纳米材料,开发用于检测Moniliformin (MON) 的敏感和快速免疫测试

Lakshani Madushika¹, Yongming Huang¹, Menghan Sun¹

¹The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

|January 22, 2025

概括

新的免疫检测方法可以检测谷物中的毒素 moniliformin (MON). 这些快速测试,包括间接竞争ELISA和免疫染色体测试条,通过在实地样本中识别MON污染来确保食品安全.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

莫尼利福明 (MON) 是一种由类动物产生的有毒代谢物.
谷物和副产品自然被MON污染,对食品安全构成重大风险.
目前,没有可用于检测现场样本中的MON的免疫测试方法.

研究的目的:

开发和验证用于检测现场样本中的 moniliformin (MON) 的免疫测试方法.
建立间接竞争性的ELISA (ic-ELISA) 和免疫染色体测试条 (ICTS) 用于MON检测.
通过快速可靠的MON污染评估,确保粮食安全.

主要方法:

开发具有高亲缘关系 (1 × 10 ^ 8) 和IgG1同型的抗MON单克隆抗体 (mAbs).
建立间接竞争型ELISA (ic-ELISA) 用于MON量化.
基于金纳米粒子 (AuNP) 和多分支金纳米花粒子 (AuNF) 的免疫染色学试验条 (ICTS) 的创建.

主要成果:

优化的ic-ELISA实现了检测极限 (LOD) 的1.55μg/mL.
基于AuNP的ICTS显示了0.83μg/mL的LOD.
基于AuNF的ICTS显示出更高的灵敏度,LOD为0.38μg/mL,并且在没有交叉反应的情况下表现出高特异性.

结论:

成功开发了免疫测试方法 (ic-ELISA和ICTS) 来检测现场样本中的MON.
开发的测试条提供快速检测,有助于提高粮食安全.
基于AuNF的ICTS的高灵敏度和特异性为常规的MON监测提供了一个有希望的工具.

关键词:

艾丽莎·伊丽莎·伊丽莎免疫染色学试验带测试带.莫尼利福尔明的使用方法单克隆抗体是一种单克隆抗体.

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