诱导的铁失调有助于通过铁亡途径对大脑内皮细胞的功能障碍
Junkyung Gil1, Donghyun Kim1, Sungbin Choi1
1College of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University ERICA Campus, Ansan, South Korea.
Toxicology and applied pharmacology
|January 22, 2025
概括
暴露会损害大脑内皮细胞,破坏血脑屏障 (BBB). 这通过线粒体功能障碍和铁过载发生,导致铁亡和大脑保护受损.
科学领域:
- 环境毒理学环境毒理学
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
背景情况:
- (Cd2+) 是一种普遍存在的环境污染物,与神经和心血管疾病有关.
- 血脑屏障 (BBB) 保护大脑,其完整性对神经健康至关重要.
- 线粒体对于通过能量和氧化还原平衡来维持BBB功能至关重要.
研究的目的:
- 研究对大脑内皮细胞的细胞毒性作用.
- 阐明引起的BBB干扰背后的机制.
- 探索线粒体和铁平衡在毒性的作用.
主要方法:
- 对bEnd.3脑内皮细胞暴露于 (Cd2+).
- 评估细胞活力,紧结蛋白表达和跨内皮电阻 (TEER).
- 评估线粒体呼吸,ATP生产,自,铁含量和脂质过氧化.
主要成果:
- 暴露降低了细胞存活率,紧结蛋白和TEER,表明BBB破坏.
- 观察到线粒体功能障碍,基本呼吸和ATP产生减少.
- 损伤的自,增加的铁含量和脂质过氧化表明铁的诱导.
结论:
- 暴露于会损害BBB的完整性和功能.
- 线粒体功能障碍和铁平衡中断是毒性的关键机制.
- 诱导的铁致死有助于BBB损伤.
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