利用人类母乳的潜力来提高纳米粒子和宏分子的肠道透性
Si Naftaly1, Topaz Pery1, Rawan Mhajne1
1Laboratory for Bioinspired Nano Engineering and Translational Therapeutics, Department of Chemical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
概括
人类母乳与牛奶或婴儿配方奶粉相比,显著增加了肠道透性,增强了纳米颗粒和宏分子的运输. 这表明营养递送和治疗干预的新应用.
科学领域:
- 生物医学科学 生物医学科学
- 营养科学 营养科学
- 材料科学 材料科学 材料科学
背景情况:
- 人类母乳 (HBM) 在婴儿营养和发育中起着至关重要的作用.
- 了解HBM与肠道屏障的相互作用是优化营养和药物输送的关键.
- 之前的研究还没有完全阐明HBM对肠道透性和纳米粒子传输的影响.
研究的目的:
- 研究HBM对肠道透性和纳米颗粒和宏分子运输的影响.
- 为了比较HBM与牛奶和婴儿配方奶粉的影响.
- 阐明HBM调节肠道屏障功能的机制.
主要方法:
- 采用肠道细胞系和猪肠道模型的体外和体外模型.
- 测量横体电阻 (TEER) 以评估屏障功能.
- 对Zonula occludens-1 (ZO-1) 蛋白质水平的分析.
- 在HBM中对脂质体-蛋白冠状病毒相互作用的评估.
主要成果:
- 与牛奶和婴儿配方奶相比,HBM显著增加了肠道透性,并降低了TEER.
- HBM降低了ZO-1水平,表明紧紧的结节的调制.
- 在猪模型中,HBM增强了脂质体的肠道透性,受蛋白质冠状结构的影响.
- 脂质体电荷的变化与HBM中改变的蛋白质冠状组合相关.
结论:
- HBM 增强了肠道的透性,促进了纳米粒子和宏分子的半细胞运输.
- HBM通过涉及紧密结合蛋白的机制调节肠道屏障功能.
- 这些发现凸显了HBM在开发营养和治疗方面的新型口服输送系统方面的潜力.
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