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在Aureobasidium pullulans YQ65中通过减少细胞内糖原含量增加了pullulan的产生

Kai Zhang¹, Lei Yue², Jingxian Cong²

¹School of Life Science, Ludong University, 186 Hongqi Road, Yantai, Shandong 264025, China; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150006, China.

Carbohydrate polymers

|January 22, 2025

概括

在Aureobasidium Pullulans中改变ApGph1基因会影响糖原和pullulan的产生. 过度表达这种基因会增加普鲁兰的产量,为工业应用提供了洞察力.

科学领域:

微生物学微生物学
生物化学生物化学
生物技术是生物技术.

背景情况:

环境pH显著影响Aureobasidium物种的生长,繁殖和碳水化合物代谢.
碳水化合物的新陈代谢,特别是糖原和外聚糖的生产,对于Aureobasidium Pullulans至关重要.

研究的目的:

在不同的pH条件下识别参与碳水化合物代谢的基因在Aureobasidium Pullulans的差异.
研究ApGph1基因 (糖原酸酶) 在调节pullulan和糖原生产中的作用.

主要方法:

在不同pH值下培养的Aureobasidium Pullulans YQ65的转录组分析.
基因淘汰 (△ApGph1) 和过度表达的ApGph1基因.
使用保存域名和Expasy工具进行分析.
料批发发酵用于评估普鲁兰产量.

主要成果:

鉴定出ApGph1基因是碳水化合物代谢中的关键参与者.
△ApGph1菌株在生长或形态上没有显著变化,但在pullulan生产和糖原含量之间表现出反向关系.
一种过度表达的ApGph1菌株通过食批发发酵在144小时内实现了42.7g/L的普卢兰产量,这与上调的普卢兰合成基因有关.

结论:

ApGph1基因是Aureobasidium Pullulans中糖原积累和pullulan生物合成之间的平衡的关键调节者.
调节ApGph1表达是一种有前途的策略,可以增强工业普鲁兰的生产.

关键词:

这就是ApGph1的意思.奥里奥巴西迪亚普卢兰斯YQ65 YQ65 葡萄糖原是什么葡萄糖原是什么普卢卢兰 (Pullulan) 是一个古老的语言.

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