相关实验视频

Updated: May 31, 2025

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

只有托尔/互乐金-1受体的基因有助于玉米的免疫反应

Qiang Zhang¹, Derong Gao¹, Lei Tian²

¹The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, Shandong Key Laboratory of Precision Molecular Crop Design and Breeding, School of Life Sciences, Shandong University, Qingdao 266237, China.

Plant physiology

|January 22, 2025

概括

玉米 (Zea mays) 的仅TIR蛋白,ZmTIR1和ZmTIR3,是由病原体诱导并增强免疫力. 它们的酶活性和通过增强疾病易感性1 (EDS1) 的信号传递对于植物对真菌感染的防御至关重要.

科学领域:

植物免疫力植物免疫力
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

托尔/互白素-1受体 (TIR) 域对于许多生物体的免疫信号传递至关重要.
像玉米一样,单种植物具有只有TIR的蛋白质,其功能在很大程度上是未知的,与典型的TIR核酸结合性白丰富重复型 (TNL) 受体不同.
玉米有三个保存的TIR-only基因:ZmTIR1,ZmTIR2和ZmTIR3.3,这些基因都是TIR基因.

研究的目的:

为了研究玉米TIR-only蛋白 (ZmTIRs) 在植物免疫中的功能.
确定ZmTIR功能背后的分子机制,包括酶活性和信号通路.
评估ZmTIRs在玉米对病原体攻击的反应中的作用.

主要方法:

全基因组扫描和玉米TIR基因的比较分析.
在Nicotiana benthamiana中进行ZmTIR1-3过度表达研究,以评估细胞死亡和防御基因诱导.
位点定向突变发生,以确定TIR功能关键残留物.
对信号分子生产的分析 (例如,2'cADPR,2',3'-cAMP,2',3'-cGMP).
一个玉米Zmtir3淘汰突变的生成和表征,该突变受到Cochliobolus heterostrophus的挑战.

主要成果:

ZmTIR1和ZmTIR3,但不是ZmTIR2,诱导了N. benthamiana的细胞死亡和防御基因表达.
在ZmTIR中,必需的谷氨酸和氨酸残留物对它们的NADase和合成酶活动以及EDS1-介导的信号传输至关重要.
ZmTIR3的过度表达产生了依赖其酶残留的信号分子 (2'cADPR,2',3'-cAMP,2',3'-cGMP).
在正常玉米中,ZmTIR的表达很低,但受到病原体的强烈诱导.
这种Zmtir3淘汰突变体对Cochliobolus heterostrophus的敏感性增加.

结论:

可诱导病原体的ZmTIR在玉米免疫力中起着重要作用.
ZmTIR的功能很可能是由它们的酶活性和EDS1-依赖的信号通路介导的.
只有TIR的蛋白质是植物防御机制的重要组成部分,特别是在单种植物中.

更多相关视频

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...