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Updated: May 13, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Published on: August 29, 2014

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评估16S-ITS-23S操作子测序对微生物群落物种级别分辨率的效率

Meghana Srinivas1,2,3, Calum J Walsh4, Fiona Crispie1,3

  • 1Teagasc Food Research Centre, Fermoy, Co. Cork, Ireland.

Scientific reports
|January 22, 2025
PubMed
概括

核糖体RNA操作子 (RRN) 测序提供了优越的微生物物种分辨率. 我们的研究发现,虽然原始对和测序平台的影响最小,但分类器和数据库的选择显著影响RRN测序准确度.

关键词:
16S rRNA 测序的测序方法在16S-ITS-23S中.牛津纳米孔技术 (ONT) 是一种这就是PacBio.在RRN操作中,RRN操作长时间阅读序列排序.

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