鉴定和表征两个功能性的抗生素MATE排泄在archaeonHalorubrum amylolyticum中的特性
Asma A Fakhoury1,2, Thomas P Thompson1, Khondaker Miraz Rahman3
1Biofilm Research Group, School of Pharmacy, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK.
npj antimicrobials and resistance
|January 22, 2025
概括
在古生物中,多种药物排放积极赋予抗菌素耐药性. 用特定化合物抑制这些可以恢复抗微生物敏感性,揭示新的耐药性机制.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 多种药物排泄是许多生物体抗微生物耐药性的关键.
- 古生物对传统的抗微生物药物表现出固有的耐药性,但机制尚不清楚.
- 哈洛鲁布鲁姆氨基 CSM52是一种具有已知的抗性特征的类古物.
研究的目的:
- 为了调查多药物和有毒化合物挤出 (MATE) 排水在Halorubrum amylolyticum CSM52.52中的存在和功能.
- 为了确定这些流出在古生物对抗菌素耐药性的作用.
- 探索克服古老抗菌素耐药性的潜在策略.
主要方法:
- 全基因组测序以识别假定的MATE排泄基因.
- 霍克斯特 33342 染料积累试验用于评估排泄活动.
- 使用和不使用排泄抑制剂 (EPI) 的抗微生物敏感性测试.
- 分子建模以了解传送器-抑制剂相互作用.
主要成果:
- 在H. amylolyticum CSM52.52中发现了两种MATE类型的排泄基因.
- 这种archaeon对广泛的抗微生物药物表现出耐药性.
- EPI显著增加了对几种抗微生物药物的敏感性,这表明活性流动.
- 分子建模表明EPI阻断了抗微生物排放.
- 遗传学分析揭示了与细菌不同的新型考古MATE.
结论:
- 这项研究提供了第一个实验证据,证明了古生物体中活跃的抗生素排泄机制.
- 马特 (MATE) 排水对H. amylolyticum的抗菌耐药性有显著的贡献.
- 了解这些古老的流出系统为打击抗菌素耐药性的新途径开辟了道路.
相关概念视频
ABC Transporters: Exporter
4.1K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
4.1K
ABC Transporters: Importer
2.7K
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
2.7K


