Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Effect of Ca<sup>2+</sup> and Mg<sup>2+</sup> on the conformational dynamics of membrane-anchored Bcl-xL.

Biochimica et biophysica acta. Biomembranes·2026
Same author

A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.

Nature methods·2026
Same author

Nanoscale Interfacial Organization Governs Maturation and Collapse in Passive versus Active Condensates.

Journal of the American Chemical Society·2026
Same author

Investigating Electron Conductivity Regimes in the Bacterial Cytochrome Wire OmcS.

The journal of physical chemistry. B·2025
Same author

Tribute to Vicki H. Grassian.

The journal of physical chemistry. A·2025
Same author

A Multimodal Adaptive Optical Microscope For <i>In Vivo</i> Imaging from Molecules to Organisms.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: May 31, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.8K

单粒子追踪显示了异质的PIEZO1扩散.

Alan T Ly1, J Alfredo Freites2, Gabriella A Bertaccini1

  • 1Department of Physiology & Biophysics, UC Irvine, Irvine, California; Sue and Bill Gross Stem Cell Research Center, UC Irvine, Irvine, California.

Biophysical journal
|January 23, 2025
PubMed
概括

机械激活的离子通道PIEZO1在活细胞中表现出异质的扩散. 膜组成和激活状态显著影响PIEZO1的移动性,揭示了其血膜动态的洞察力.

更多相关视频

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.2K
Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels

Published on: September 8, 2016

10.2K

相关实验视频

Last Updated: May 31, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.8K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.2K
Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels

Published on: September 8, 2016

10.2K

科学领域:

  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.
  • 膜蛋白的动力学 膜蛋白的动力学

背景情况:

  • 机械激活的离子通道PIEZO1在各种生理过程中起着至关重要的作用.
  • PIEZO1是由机械线索激活的,其在等离子体膜中的移动性对其功能至关重要.

研究的目的:

  • 在活细胞中研究内源PIEZO1的扩散动态.
  • 确定血组成和通道激活如何影响PIEZO1的移动性.

主要方法:

  • 使用全内部反射光显微镜对 tdTomato 标记的 PIEZO1 进行单颗粒追踪 (SPT).
  • 操纵膜胆固醇水平,并添加马加里酸.
  • 用PIEZO1激动剂Yoda1和抑制剂GsMTx-4进行治疗.

主要成果:

  • PIEZO1显示异质扩散,分为移动和不移动的子群.
  • 胆固醇消耗和Yoda1治疗增加了PIEZO1的移动性.
  • GsMTx-4治疗和胆固醇补充剂降低了PIEZO1的移动性.
  • 移动PIEZO1点点表现出异常的亚扩散.

结论:

  • 血的组成和PIEZO1的激活状态极大地调节了它的扩散.
  • 了解PIEZO1的移动性是阐明它在细胞机械转导中的作用的关键.
  • 这些发现为研究细胞过程如何影响PIEZO1活动提供了基础.