通过发酵优化红醇的生产,使用糖蜜作为碳源
Riahna Kembaren1, Arli Aditya Parikesit1, Jocelyn Nataniel1
1Department of Biotechnology, Indonesia International Institute for Life Sciences, East Jakarta, Indonesia.
Acta biochimica Polonica
|January 23, 2025
概括
优化发酵条件,包括糖蜜,酵母提取物和NaCl度,大大提高了红醇的产生. 联储批发发酵进一步提高了产量,为这种糖替代品的工业应用提供了一个有前途的战略.
科学领域:
- 生物技术和发酵科学 生物技术和发酵科学
- 食品科学与技术 食品科学与技术
- 代谢工程是代谢工程.
背景情况:
- 红醇是一种糖醇,是糖尿病患者的有价值的糖替代品.
- 微生物的红醇产生受透压,压力和营养物质的可用性影响.
- 优化碳源,源和它们的比率对于高效的红醇生物合成至关重要.
研究的目的:
- 为了确定糖蜜 (碳源) 和酵母提取物 (源) 的最佳度,以生产红醇.
- 为了研究碳与 (C/N) 比率,NaCl度和pH对红醇产量的影响.
- 为了比较批量和料批量发酵系统的有效性,以最大限度地提高红醇生产率.
主要方法:
- 使用一次性一个因素 (OFAT) 方法系统地优化生产参数.
- 测试了不同度的糖蜜,酵母提取物,NaCl和pH值.
- 批量和食批量发酵策略在红醇产量和生产率方面进行了比较.
主要成果:
- 批发发酵的最佳条件包括200g/L糖蜜,7g/L酵母提取物 (C/N比为200/7),和25g/LNaCl,产生17.48g/L红醇.
- 红醇生产的最佳pH值被确定为5.
- 料批发发酵显著提高了红醇度至26.52g/L,提高了产量 (0.501g/g) 和生产力 (0.158g/Lh).
结论:
- 优化碳和来源,NaCl度和pH值对于最大限度地提高红醇生产至关重要.
- 与批次相比,料批次发酵是一种优越的系统,可以实现较高的红醇产量和生产率.
- 这些发现为可扩展的红醇工业生产提供了基础.
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