循环的miR-29c,miR-195和miR-486是确定适度强度抵抗运动的客观指标
Daisuke Takamura1,2, Kentaro Iwata1, Shota Inoue2
1Department of Rehabilitation, Kobe City Medical Center General Hospital, Kobe, JPN.
Cureus
|January 23, 2025
概括
新的生物标志物,包括miR-486,可以客观地表明有效的抵抗运动强度. 这些微RNA可能有助于预测个体对运动训练的反应.
科学领域:
- 运动生理学 运动生理学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 适度运动对健康至关重要,但客观地定义其强度仍然具有挑战性.
- 对运动反应的个体变化需要有效强度的可靠指标.
研究的目的:
- 为了确定新的客观生物标志物来确定有效的运动强度.
- 为了研究微RNA表达的变化,以应对不同强度的运动在人类和动物.
主要方法:
- 对在小鼠中循环的微RNA进行微阵列分析,这些小鼠接受了有效的强度运动.
- 定量实时PCR用于评估在有氧和抵抗运动方案后人类的microRNA变化.
- 人类研究涉及四周的低强度 (LI) 和高强度 (HI) 运动组.
主要成果:
- 微阵列分析显示,在有氧和抵抗运动后,microRNA表达的显著变化.
- 确定了miR-29c,miR-23b,miR-222,miR-195,miR-126,miR-133a和miR-486作为候选生物标志物.
- 在人类中,高强度阻力运动导致身体性能改善和miR-29c,miR-195,miR-486的表达增加,miR-486折叠变化与膝盖延伸器强度增加相关.
结论:
- 特定的微RNAs,即miR-29c,miR-195和miR-486,通过有效的强度抵抗运动进行上调.
- 这些microRNA显示出作为评估抵抗运动强度的客观指标的潜力.
- miR-486可以作为一个预测生物标志物来预测个体对抵抗运动训练的反应.
相关概念视频
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...