高胆固醇血症会引发天生的免疫失衡,并在缺血性中风后改变脑梗塞
Ali Ata Tuz1, Nils Hoerenbaum1, Özgür Ulusoy1
1Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
高胆固醇 (高胆固醇血症) 通过增加中性粒细胞激活和神经炎症,使中风的结果变得更糟. 这项研究表明,高胆固醇血会加剧中风引起的免疫反应和脑损伤.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 心血管研究研究心血管研究
背景情况:
- 脑卒中后的中性粒细胞激活导致神经炎症和不良结果.
- 高胆固醇血症是一种先前存在的疾病,可能会影响中风期间的免疫细胞反应.
研究的目的:
- 研究饮食诱导的高胆固醇贫血对小鼠早期中性粒细胞激活和中风结果的影响.
主要方法:
- 在引发中风之前,小鼠被食高脂肪饮食 (HFD) 或正常饮食 (ND) 六周.
- 流细胞计分析了免疫细胞激活标记物和血细胞因子.
- 用ELISA量化了中性粒细胞外细胞陷 (NETs).
主要成果:
- 在中风后,HFD小鼠表现出循环中性粒细胞的增加,中性粒细胞激活标记的升高 (Ly6G,PSGL-1),以及单细胞/巨细胞 (MΦ) 激活的升高 (Ly6C,CD68).
- 高血压导致高血水平的促炎性细胞因子 (TNF-α,IL-6,IL-23,MCP-1) 和增加的NETs.
- 与ND小鼠相比,HFD小鼠在大脑中显示出更大的中性粒细胞透和更大的心脏病发作体积.
结论:
- 高胆固醇血症显著增强中性粒细胞和MΦ在中风后的早期激活.
- 在高胆固醇血症条件下,这种增强的免疫反应加剧了中风引起的神经炎症,并加剧了疾病的结果.
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