相关实验视频

Updated: May 31, 2025

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

在11q异常的高等级B细胞淋巴瘤中微RNA表达

Gioia Di Stefano^1,2, Anja Fischer², Emil Chteinberg²

¹Histopathology and Molecular Diagnostics, Careggi University Hospital, Florence, Italy.

Genes, chromosomes & cancer

|January 23, 2025

概括

与伯基特淋巴瘤 (BL) 和扩散型大B细胞淋巴瘤 (DLBCL) 相比,具有11q偏差的高度B细胞淋巴瘤 (HGBCL-11q) 显示出明显的microRNA (miRNA) 表达. miR-3919在HGBCL-11q中显著过度表达,提供了潜在的诊断见解.

科学领域:

血液学血液学血液学
在瘤学瘤学.
分子生物学分子生物学

背景情况:

成熟的攻击性B细胞淋巴瘤,包括伯基特淋巴瘤 (BL) 和扩散性大B细胞淋巴瘤 (DLBCL),表现出可变的微RNA (miRNA) 表达.
高度B细胞淋巴瘤与11q异常 (HGBCL-11q) 与BL和DLBCL具有生物学相似性,但其miRNA特征未得到充分研究.

研究的目的:

研究和比较HGBCL-11q与BL和DLBCL的miRNA表达特征.
识别特定的miRNAs,使HGBCL-11q与其他攻击性B细胞淋巴瘤区分开来.

主要方法:

在HGBCL-11q,BL,DLBCL和桃体样本中分析了2083个miRNA的表达特征.
使用HTG EdgeSeq miRNA全转录组测定用于miRNA分析.
使用了统一的多重近似和投影 (UMAP) 和DESeq2差异表达分析.

主要成果:

对于淋巴瘤,UMAP分析没有显示出明显的miRNA表达集群.
与BL和DLBCL相比,差异表达分析在HGBCL-11q中确定了特定的上调小RNA.
与BL和DLBCL相比,miR-3919在HGBCL-11q中持续且显著过度表达 (p < 0.001).

结论:

与BL和DLBCL相比,HGBCL-11q具有独特的miRNA表达特征.
鉴定出差异表达的miRNAs,特别是miR-3919,可能作为HGBCL-11q.的潜在生物标志物.
对这些miRNA差异的进一步研究可以完善淋巴瘤分类和治疗策略.

关键词:

伯基特淋巴瘤是一种淋巴瘤扩散的大B细胞淋巴瘤高度B细胞淋巴瘤与11q异常.微型RNAs是什么意思

更多相关视频

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...