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鉴定了新的动物和形骨科病毒,揭示了E型肝炎病毒的演变

Fu-Li Li^1,2, Bo Wang³, Pei-Yu Han¹

¹Institute of Preventive Medicine, School of Public Health, Dali University, Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from Western Yunnan, Yunnan Key Laboratory of Zoonotic Disease Cross-border Prevention and Quarantine, Dali, Yunnan 671000, China.

Zoological research

|January 23, 2025

概括

研究人员在中国对野生小哺乳动物进行了肝炎E病毒 (HEV) 的查,并发现了多种不同的Orthohepevirus菌株. 这些发现揭示了新的宿主物种,并突出了HEV进化中的共同物种化和跨物种传播.

科学领域:

病毒学病毒学
进化生物学进化生物学
动物传播疾病动物传播疾病

背景情况:

型病毒家族,包括肝炎E病毒 (HEV),呈现出快速扩张的宿主范围.
鼠类HEV的出现带来了动物传播的风险.
对于HEV的进化路径,人们对其了解甚少.

研究的目的:

在中国云南省野生小型哺乳动物中调查Orthohepevirus的流行率和遗传多样性.
为了确定HEV的新宿主物种.
为了阐明HEV在小型哺乳动物种群中的进化动态.

主要方法:

在37种野生小哺乳动物中对2464种野生小哺乳动物进行了系统查,使用广泛反应的反转录聚合酶链反应 (RT-PCR).
下一代测序以获得全长的病毒基因组.
遗传学和序列分析以确定病毒多样性和进化关系.

主要成果:

确定了来自10个物种的192个阳性标本 (检测率为7.79%).
从8个物种中找到了24个全长基因组序列,其中包括新的宿主物种.
观察到广泛的遗传多样性,菌株分为罗卡赫佩病毒拉蒂C1,C3和罗卡赫佩病毒eothenomi,以及一个未分类的分离菌株.

结论:

野生小型哺乳动物携带多种多样的Orthohepevirus菌株,扩大已知的HEV宿主范围.
遗传学分析表明,HEV与宿主种群之间存在强烈的关联,这表明共同物种化.
跨物种传播在塑造Paslahepevirus和Rocahepevirus属的演变中发挥着重要作用.

关键词:

遗传多样性遗传多样性基因组特征的鉴定肝炎E病毒 (HEV) 是一种病毒.分子进化分子进化骨科肝炎病毒 (Orthohepevirus) 是一种病毒.罗卡赫佩病毒 (HEV-C) 病毒动物动物一个子.

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