PRRSV-2 变种分类:用于加强监测和监视的动态分类
Kimberly VanderWaal1, Nakarin Pamornchainavakul1, Mariana Kikuti1
1Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA.
mSphere
|January 23, 2025
概括
猪生殖和呼吸系统综合征病毒2型 (PRRSV-2) 变种的新分类系统为兽医提供了一种高分辨率的工具,用于跟踪病毒演变. 该系统增强了流行病学监测和行业对新出现的PRRSV-2遗传变异的反应.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 猪病监测 猪疾病监测
背景情况:
- 现有的PRRSV-2遗传分类系统缺乏有效的流行病学监测所需的分辨率.
- 亚系和其他方法在确定密切的遗传关系或实时跟踪病毒演变方面不可靠.
研究的目的:
- 在美国开发和验证PRRSV-2遗传变异的细分分类系统.
- 为基于开放式读取5 (ORF5) 序列的PRRSV-2进行分类提供一种可靠,可复制和可适应的方法.
- 创建一个公开可访问的网络工具,用于为已识别的变种分配新的序列.
主要方法:
- 集群算法适用于2015-2023年超过25,000个美国PRRSV-2 ORF5序列.
- 每三个月对新序列进行系统分类,以识别新出现的变异.
- 开发一个用于实时变种识别和分类的网络工具.
主要成果:
- 在2015年至2023年间,确定了118种PRRSV-2变种,每年约有48种活跃变种.
- 变体内基因距离的平均值为2.4%,与最近相关变体的平均距离为4.9%.
- 该分类系统展示了强大的和可重复的结果,动态地容纳新的遗传多样性.
结论:
- 与现有方法相比,精细的PRRSV-2变种分类系统提供了更好的分辨率和适应性.
- 该系统和相关的网络工具提高了区分菌株,识别引入来源和跟踪新兴变异的能力.
- 该系统的采用将大大提高PRRSV-2的流行病监测,研究和行业应对策略.
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