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CD209d/e在流感病毒感染期间促进炎症和肺损伤
Radha Gopal1, Michael A Marinelli1, Flavia Rago1
1Division of Pulmonary Medicine, Department of Pediatrics, UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA, United States.
ImmunoHorizons
|January 23, 2025
概括
缺乏CD209d/e的小鼠显示流感病毒载量降低和肺炎. 这表明CD209d/e对于感知和响应流感病毒感染至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 流感病毒导致全球显著的发病率和死亡率.
- 模式识别受体 (PRRs) 调解宿主对病毒感染的免疫反应.
- 包括DC-SIGN同类在内的CD209蛋白家族可能会影响抗病毒免疫力,但它们在流感中的作用尚不清楚.
研究的目的:
- 为了研究小鼠CD209d/e同类在流感病毒感染期间肺部免疫反应中的作用.
- 为了确定CD209d/e缺乏是否影响病毒清除,免疫细胞透和肺部炎症信号.
主要方法:
- 生成的CD209d/e基因缺陷 (CD209d/e-/-) 的小鼠.
- 感染流感病毒的野生型和CD209d/e-/-小鼠.
- 评估了支气管支气管洗细胞性,细胞因子和抗病毒基因表达,通类受体 (TLR) 水平和肺病理.
- 在隔离的巨细胞中评估病毒吸收和体外对TLR激动剂的反应.
主要成果:
- CD209d/e-/-小鼠的病毒载量降低,TLR3和TLR9的表达率降低,干扰素反应降低.
- 在CD209d/e-/-小鼠的支气管支气管洗中存在较少的巨细胞,并减少了对酶体肺炎的发生.
- 缺少CD209d/e导致膜和骨髓衍生的巨细胞中流感病毒吸收减少.
- 缺乏CD209d/e在TLR激动剂刺激时降低了TLR3,TLR9,RIG1,STAT1和STAT2的表达.
结论:
- CD209d/e在宿主感知和吸收流感病毒方面发挥着重要作用.
- CD209d/e对于对流感病毒感染进行有效的炎症性免疫反应很重要.
- 向CD209d/e可能提供一种策略,在流感病毒感染期间调节免疫反应.
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