用液体染色学高分辨率质谱仪在马尿和血中含有兴奋剂的选分析与稀释和射击
Eylem Funda Göktaş1, Erol Kabil1, Esma Söylemez Yeşilçimen2,3
1Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, 34890, Istanbul, Türkiye. eylemfundagoktas@gmail.com.
The Analyst
|January 23, 2025
概括
一种新的高性能液态染色体质谱法方法快速选马样中的200多种禁止物质. 这种具有成本效益的稀释和射击协议为兴奋剂控制提供了广泛的频谱分析.
科学领域:
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
- 兽医科学 兽医科学 兽医科学
背景情况:
- 在生物样本中精确检测各种药理物质至关重要.
- 实验室需要高效,具有成本效益的方法来进行大量的多因素分析.
- 需要简单的样品制备方法,快速分析和稳定性.
研究的目的:
- 开发和验证马匹生物样本中禁止物质的高通量选协议.
- 识别可直接分析而无需水解的化合物,具有短期稳定性.
- 建立一个具有成本效益的兴奋剂分析方法.
主要方法:
- 利用高性能液态色谱学与高分辨率质谱学 (HPLC-HRMS) 相结合.
- 开发了一种用于马尿和血样本的"稀释和射击"协议.
- 使用C18列,具有特定的移动相梯度,并根据2002/657/EC验证.
主要成果:
- 在13分钟的运行时间内,成功选了多个类别的200多种禁止物质.
- 目标物质的检测能力从≤1到200 ng/mL不等.
- 通过对真实阳性病例样本的兴奋剂分析来证明其实际适用性.
结论:
- 开发的HPLC-HRMS方法对于禁止物质的广泛分析是快速的,实用的和具有成本效益的.
- 该方法适用于马的尿液和血样本,这是一个罕见的能力.
- 这种高通量选协议显著提高了兴奋剂控制效率.
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