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相关概念视频

Cooperative Binding of Transcription Regulators02:13

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Cis-regulatory Sequences02:02

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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相关实验视频

Updated: May 31, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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编码关系链接RNA G-四重复合体和蛋白质RGG动机在RNA结合蛋白自调节中.

Marlene Adlhart1,2, Daniel Hoffmann1,2, Anton A Polyansky1,2

  • 1Max Perutz Labs, Vienna Biocenter Campus, Vienna 1030, Austria.

Proceedings of the National Academy of Sciences of the United States of America
|January 23, 2025
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概括

富含关氨酸的RNA G四复合体 (rG4s) 可以在信使RNA (mRNA) 中编码富含氨酸和糖氨酸的动机 (RGG动机). 这种遗传密码特征将RNA结构与蛋白质结合联系起来,可能调节基因表达.

关键词:
这是一个RGG图案.在RNA G-四重复合体中.RNA结合蛋白质是RNA结合的蛋白质.自主监管是自主监管的一个方面.遗传代码是一种遗传代码.

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相关实验视频

Last Updated: May 31, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 富含关氨酸的RNA序列形成G-四复合体 (rG4s).
  • 具有氨酸-糖氨酸丰富基因 (RGG基因) 的RNA结合蛋白 (RBPs) 识别rG4s.
  • 氨酸和甘氨酸密码子是富含关氨酸的.

研究的目的:

  • 为了研究rG4s和RGG动机编码在自身信使RNA (mRNAs) 之间的关系.
  • 要确定这种关系是否在遗传密码中固有,并影响蛋白质-RNA相互作用.
  • 确定涉及这些相互作用的特定RBP.

主要方法:

  • 对rG4数据集的全转录组分析.
  • 使用随机遗传代码来评估编码关系.
  • 增强交叉连接和免疫沉降 (eCLIP) 数据的分析.

主要成果:

  • 数以百计的人类RGG图案被rG4s编码,特别是较长的图案.
  • rG4/RGG编码关系是通用遗传密码的结果.
  • 具有rG4编码的RGG基因的蛋白质显示了增加的RNA结合.
  • 特定的RBP (FUS,FMRP,G3BP1) 与这些部位的自身mRNA相互作用.

结论:

  • 存在一种机制,即RNA结构 (rG4s) 决定了同一mRNA中的蛋白结合基因 (RGG) 编码.
  • 这种RNA G-quadruplex/RGG图案编码嵌入在遗传码中.
  • 这种相互作用可能为基因表达控制建立自我调节的反循环.