编码关系链接RNA G-四重复合体和蛋白质RGG动机在RNA结合蛋白自调节中
Marlene Adlhart1,2, Daniel Hoffmann1,2, Anton A Polyansky1,2
1Max Perutz Labs, Vienna Biocenter Campus, Vienna 1030, Austria.
概括
富含关氨酸的RNA G四复合体 (rG4s) 可以在信使RNA (mRNA) 中编码富含氨酸和糖氨酸的动机 (RGG动机). 这种遗传密码特征将RNA结构与蛋白质结合联系起来,可能调节基因表达.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 富含关氨酸的RNA序列形成G-四复合体 (rG4s).
- 具有氨酸-糖氨酸丰富基因 (RGG基因) 的RNA结合蛋白 (RBPs) 识别rG4s.
- 氨酸和甘氨酸密码子是富含关氨酸的.
研究的目的:
- 为了研究rG4s和RGG动机编码在自身信使RNA (mRNAs) 之间的关系.
- 要确定这种关系是否在遗传密码中固有,并影响蛋白质-RNA相互作用.
- 确定涉及这些相互作用的特定RBP.
主要方法:
- 对rG4数据集的全转录组分析.
- 使用随机遗传代码来评估编码关系.
- 增强交叉连接和免疫沉降 (eCLIP) 数据的分析.
主要成果:
- 数以百计的人类RGG图案被rG4s编码,特别是较长的图案.
- rG4/RGG编码关系是通用遗传密码的结果.
- 具有rG4编码的RGG基因的蛋白质显示了增加的RNA结合.
- 特定的RBP (FUS,FMRP,G3BP1) 与这些部位的自身mRNA相互作用.
结论:
- 存在一种机制,即RNA结构 (rG4s) 决定了同一mRNA中的蛋白结合基因 (RGG) 编码.
- 这种RNA G-quadruplex/RGG图案编码嵌入在遗传码中.
- 这种相互作用可能为基因表达控制建立自我调节的反循环.
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