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登革热病毒血型和基因型的鉴定:比哈尔邦帕特纳艾滋病医院的一项综合研究

Kamal Singh¹, Md Zeeshan Farooque¹, Vikram Pal Gandhi¹

¹Regional Viral Research and Diagnostic Laboratory, Department of Microbiology, All India Institute of Medical Sciences, Patna, Bihar, India.

Indian journal of medical microbiology

|January 23, 2025

概括

这项研究确定了2021年该地区传播的四种登革热病毒 (DENV) 血清型,其中DENV-2是最常见的. 基因型鉴定揭示了每个血清型的特定基因型集群,为登革热流行病学提供了关键的见解.

科学领域:

病毒学病毒学
流行病学流行病学
公共卫生公共卫生

背景情况:

登革热病毒 (DENV) 由于其遗传多样性,造成多种血清型和基因型,构成了全球健康的重大威胁.
蚊子传播的疾病,如登革热,正在越来越多地将传播模式从城市转移到郊区和农村地区.
了解循环DENV血清型和基因型对于有效的疾病控制和预防策略至关重要.

研究的目的:

确定和描述2021年在特定地区流行的登革热病毒血清型和基因型.
提供有关登革热病毒流通的最新流行病学数据.

主要方法:

在收集的血清样本上进行了血清分析 (通过ELISA检测NS1抗原和IgM抗体).
实时RT-PCR用于登革热血型鉴定.
针对囊前膜 (C-prM) 区域的常规PCR被用于登革热病毒基因定型.

主要成果:

在933个血清样本中,75个检测出NS1抗原,135个检测出登革热IgM,结果呈阳性.
实时RT-PCR检测了所有四种DENV血清型:DEN-1 (7.8%),DEN-2 (50.9%),DEN-3 (39%) 和DEN-4 (1.9%).
33个样本的基因型检测显示DENV-1与GIII,DENV-2与IVa,IVb和IVc基因型,DENV-3与GIII,DENV-4与GIII.

结论:

这项研究提供了关于流通的登革热病毒血清型及其在研究区域中普遍存在的基因型的最新见解.
这些发现突出了多个DENV血清型和特定基因型分布的共同循环,为公共卫生干预提供了信息.

关键词:

登革热病毒 (DENV) 是一种病毒.登革热病毒血清型和基因型弗拉维病毒 (flavivirus) 是一种病毒.

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