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鉴定血清中小型非编码RNA作为子宫内膜受体性的生物标志物

Shaoyuan Xu¹, Dongling Hu², Yanqin Ye²

¹Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China; Reproductive Medicine Center, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China; Hubei Clinical Research Center for Reproductive Medicine, Shiyan, Hubei 442000, China.

|January 23, 2025

概括

血清小非编码RNAs (sncRNAs) 显示为对子宫内膜受体测试的非侵入性生物标志物具有前途. 血清中的四种特定的tsRNA可以准确地预测子宫内膜接受度,为生育治疗提供一种不那么侵入性的替代方案.

科学领域:

生殖生物学生殖生物学
分子诊断学分子诊断学
基因组学就是基因组学.

背景情况:

目前的子宫内膜受体分析 (ERA) 是侵入性的,阻碍了同一周期的胚胎移植.
需要使用非侵入性生物标志物来评估子宫内膜受体性.

研究的目的:

选血清小非编码RNAs (sncRNAs) 作为ERA测试的非侵入性生物标志物.
调查tsRNA-35:73-Asp-GTC-1在子宫内膜受体性中的作用.

主要方法:

从12名接受IVF-ET和ERA的不育患者的血清样本中,使用高能测序分析了sncRNA配置文件.
tsRNA-35:73-Asp-GTC-1在人类子宫内膜层细胞 (HESC) 模型中被过度表达并被击倒,以研究其对决定化的影响.
使用RT-qPCR和PCR分析进行了目标基因验证.

主要成果:

鉴定了286个差异表达的tsRNA,46个miRNA和106个piRNA.
通过ROC曲线分析,四个血清tsRNA显示出对子宫内膜接受性的良好预测价值.
tsRNA-35:73-Asp-GTC-1 影响了落叶细胞形态和指标,并与Wnt3目标基因相关联.

结论:

血清sncRNA分析提供了一种潜在的非侵入性方法来评估子宫内膜受体性.
四个血清tsRNA可以作为用于非侵入性ERA检测的新生物标志物.
TsRNA-35:73-Asp-GTC-1可能通过向Wnt3.3来调节子宫内膜受体.

关键词:

宫内膜的受体性宫内膜的受体性试管婴儿-ETET,这是一个非常好的方法.植入植入的植入窗口的植入窗口.这就是SncRNA.这是一个 TsRNA TsRNA.

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