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通过在发芽的酵母菌中发生感觉/反感觉转录的重叠而形成双链RNA的普遍形成

Ugo Szachnowski¹, Emmanuelle Becker², Igor Stuparević²

¹Institut Curie, Sorbonne Université, CNRS UMR3244, F-75248 Paris, France.

RNA (New York, N.Y.)

|January 23, 2025

概括

这项研究确定了酵母中的347个位点的双链RNA (dsRNA),揭示了它们在全基因组的流行率和基因表达中的调节作用. 一个新的工具,SensR查看器,有助于探索这些dsRNA和RNA表达模式.

科学领域:

分子生物学分子生物学
遗传学遗传学是一种遗传学.
生物信息学是一种生物信息学.

背景情况:

已知重叠的感觉/反感觉 (s/a) 转录在各种生物体中形成双链RNA (dsRNA).
在生长和发育期间,双胞胎发芽酵母中dsRNA形成的全基因组流行率和功能意义在很大程度上仍未被探索.

研究的目的:

为了研究dSRNAs在*Saccharomyces cerevisiae*中的全基因组发生情况.
探索dsrna形成在不同生命周期阶段调节转录和蛋白质水平的功能影响.
开发一种可视化dsRNA和RNA表达数据的工具.

主要方法:

使用了一种*Saccharomyces cerevisiae*菌株,该菌株与特定的核糖核酶和RNA结合蛋白基因进行了工程.
监测了双胞胎生命周期 (线粒分裂和半分裂) 的不同阶段的dsRNA形成.
开发了用于数据可视化的感觉/反感觉双链RNA (SensR) 表达式查看器.

主要成果:

在347个s/a位点确定了dSRNAs,表现出部分或完全的转录重叠.
与先前特征的反感干扰位点和调节蛋白质水平的相关dsrnas.
证明了dsRNAs在双胞胎酵母菌的线粒分裂和半分裂中的作用.

结论:

dsRNA形成是双胞胎发芽酵母中普遍存在的现象,影响基因调节.
这些发现有助于改善酵母基因组注释和对基于RNA的调节机制的理解.
该研究提供了对跨物种保存的dsRNA形成的进化见解.

关键词:

反意义的成绩单的成绩单这是一种双链RNA.长非编码RNA是什么意思酵母酵母是一种酵母.

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