人类乳头瘤病毒口腔感染个体的微生物组分析
David Israel Escobar Marcillo1, Grete Francesca Privitera2, Francesca Rollo3
1Section of Mechanisms, Biomarkers and Models, Department of Environment and Health, Istituto Superiore di Sanità, Rome, Italy.
Scientific reports
|January 23, 2025
概括
口腔微生物群在患有或没有高风险人类乳头瘤病毒 (hrHPV) 感染的个体之间存在差异,特别是在没有艾滋病毒的人群中. 这表明 hrHPV,口腔细菌和艾滋病毒状态在癌症发展中的相互作用.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
背景情况:
- 人类微生物组在上皮癌中发挥作用.
- 高风险的人类乳头瘤病毒 (hrHPV) 感染与口腔状细胞癌有关,但仅在受感染个体的一个子集中.
- 其他因素可能会影响 hrHPV 感染后的癌症发展.
研究的目的:
- 与对照组相比,研究 hrHPV 感染的个体的口腔微生物组合.
- 探索基于艾滋病毒状况的潜在差异.
主要方法:
- 16S rRNA基因测序来自 hrHPV阳性和HPV阴性受试者的口腔冲洗和嘴样本.
- 对微生物差异和途径丰度的分析,按艾滋病毒状况分层.
- 阿尔法和β多样性指标的比较.
主要成果:
- 在 hrHPV 阳性和 HPV 阴性受试者之间观察到口腔微生物群多样性的显著差异 (alpha 和 beta),但仅在 HIV 阴性个体中.
- hrHPV感染改变了特定细菌的丰富性:HIV阳性人群中的Sacharibacteria和HIV阴性人群中的Gracilibacteria.
- 基因层面的分析显示,HRHPV阳性HIV阳性受试者中Bulleidia的丰富,HRHPV阳性HIV阴性受试者中Parvimonas的丰富和Alloscardovia的枯竭.
结论:
- 口腔微生物组合与 hrHPV 感染有关.
- hrHPV与口腔微生物群之间的相互作用可能因艾滋病毒状况而受到调节.
- 需要进一步的研究,以了解这些相互作用在口腔癌发展中的作用.
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