从大豆和豆中可持续提取植物性雌激素,使用绿色溶剂
Ramon S B Ferreira1, Patrícia T de Souza1, Luana P Cardoso2
1Laboratório de Extração, Termodinâmica Aplicada e Equilíbrio - EXTRAE, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas, Rua Monteiro Lobato,80, 13083-062 Campinas, SP, Brazil.
Food research international (Ottawa, Ont.)
|January 24, 2025
概括
这项研究开发了一种使用深性溶剂 (DES) 来回收大豆废物 (okara) 的异黄的绿色提取方法. 优化的DES方法有效提取这些有益的化合物,为传统溶剂提供可持续的替代品.
科学领域:
- 绿色化学 绿色化学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 作为大豆加工的副产品,OKARA含有丰富的异黄,这些有价值的生物活性化合物具有潜在的健康益处.
- 传统的提取方法经常使用有毒溶剂,造成环境和健康问题.
- 开发可持续和高效的方法来从中回收异黄对于价值化至关重要.
研究的目的:
- 为了优化从奥卡拉和大豆中提取异黄的提取,使用深层欧特基溶剂 (DES).
- 评估DES在异黄回收方面的效率和可持续性.
- 评估提取的化合物的细胞毒性,并优化安全的过程.
主要方法:
- 使用分数设计和中央复合可旋设计 (CCRD) 选提取变量 (温度,水含量,固体-液体比,速度).
- 使用由胆化物 ([Ch]Cl) 和酸 (AA) 组成的深度欧特基溶剂 (DES).
- 在Caco-2细胞上进行细胞毒性测试,并使用扫描电子显微镜 (SEM) 评估固体矩阵.
主要成果:
- 使用特定的DES条件,优化从大豆中提取1194.56μg TIC/g和从花中提取450.9μg TIC/g.
- 确定了最佳温度,水百分比,固体液体比率和速度,以有效提取异黄.
- 细胞毒性测试表明,需要中和酸性DES提取物以减轻不良影响.
结论:
- DES的水性混合物为从大豆残留物 (okara) 中回收异黄提供了可持续和有效的替代方案.
- 这种绿色方法可以替代异黄提取中的危险常规溶剂.
- 这项研究表明,通过高效和环保的生物活性化合物回收来提升okara的潜力.
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