用于向MRSA病毒性的Micromeria barbata:在和体外研究
Shiraz Rawas1, Nawal Al Hakawati1, Amale Mcheik2
1Department of Biological Sciences, Faculty of Science, Beirut Arab University, Tripoli, 1300, Lebanon.
Heliyon
|January 24, 2025
概括
来自Micromeria barbata树叶和茎的乙醇提取物显示出对抗甲林耐药黄金葡萄球菌 (MRSA) 的显著抗菌活性. 叶子提取物在抑制MRSA生长和生物膜形成方面表现出更强的结合亲和力和更大的有效性.
科学领域:
- 植物化学 植物化学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 甲素耐药黄金葡萄球菌 (MRSA) 由于其对常规抗生素的耐药性,构成了严重的全球健康威胁.
- 自然产品,如植物提取物,正在越来越多地研究新型抗菌剂.
- 麦克罗梅里亚巴巴塔 (Micromeria barbata) 是一种具有传统使用历史的药用植物,需要对其生物活性化合物的科学研究.
研究的目的:
- 来自Micromeria barbata叶 (LE) 和茎 (SE) 的乙醇提取物的植物化学含量.
- 评估这些提取物对MRSA的抗菌活性.
- 探索M. barbata提取物作为新抗菌治疗的来源的潜力.
主要方法:
- 使用里埃变换红外光谱 (FTIR) 和气色谱-质谱 (GC-MS) 的植物化学分析.
- 分子对接模拟以评估已识别的化合物与MRSA蛋白 (SarA和CrtM) 的结合亲和力.
- 抗微生物测定包括阿加井扩散,最小抑制度 (MIC),最小杀菌度 (MBC) 和生物膜抑制测定.
主要成果:
- FTIR确定了基,基,胺和芳香的功能组. 在GC-MS中发现了九种不同的化合物,包括SE中的piperitenone,cyclohexene,palmitic acid,safranal和oleic acid,LE中的iso-menthone,oleic acid,1-pentanol,kumarone和.
- 与SE化合物相比,分子对接表明LE化合物与SarA蛋白的结合亲和力更强 (ΔG值-10.9至-11.6).
- SE和LE都表现出对MRSA的显著抗菌活性,抑制区分别为15毫米和20毫米. LE显示MIC值 (6.25 mg/ml) 比SE值 (12.5 mg/ml) 低,并且在生物膜形成和杀菌活性方面有较大的度依赖性减少.
结论:
- 微细菌巴巴塔的乙醇提取物,特别是从叶子中提取的,对MRSA具有显著的抗菌特性.
- 鉴定到的植物化学物质及其结合亲和性表明了抗菌作用的机制.
- M. barbata 提取物作为开发针对MRSA感染的新型治疗策略的潜在来源显示出希望.
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