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福莫诺尼丁促进了猪卵细胞的成熟,并通过减少氧化应激来改善胚胎发育

Na Wang¹, Han Yang¹, Yelei Chen¹

¹Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China.

Frontiers in cell and developmental biology

|January 24, 2025

概括

一种抗氧化剂黄胺 (FMN) 的形式诺尼丁 (Formononetin) 通过减少氧化应激和增强线粒体功能来改善体外卵细胞成熟和早期胚胎发育. 这项研究强调了FMN.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
生物化学生物化学

背景情况:

氧化应激对卵细胞成熟和胚胎发育产生负面影响.
抗氧化剂通常在体外用于改善这些过程.
甲诺丁 (FMN) 是一种具有已知的抗氧化特性的黄类.

研究的目的:

研究FMN作为抗氧化剂对体外卵细胞和早期胚胎发育的影响.
为了确定FMN补充是否可以提高卵细胞成熟率和胚胎细胞形成率.

主要方法:

在体外培养系统中补充0.5μM的FMN.
评估卵细胞成熟 (第一个极体挤出) 和胚胎细胞形成率.
测量线粒体功能,ATP水平,活性氧物种 (ROS) 和谷氨 (GSH) 水平.
评估胚胎囊细胞的自和亡,可能通过Nrf2/Keap1通路.

主要成果:

补充FMN显著提高了第一极体挤出和胚胎细胞形成率.
随着FMN治疗,观察到线粒体功能和ATP水平的增加.
卵细胞和胚胎中的细胞内ROS降低和细胞内GSH水平升高.
囊胚细胞中自和亡的减少,表明Nrf2/Keap1通路的调节.

结论:

以0.5μM的FMN补充在体外卵细胞和早期胚胎发育方面有好处.
FMN充当抗氧化剂,改善卵细胞和胚胎质量.
潜在的机制包括通过Nrf2/Keap1通路调节氧化应激,线粒体功能和自.

关键词:

Nrf2/Keap1 的第一名.自自是自的过程.对于单尼的使用.线粒体功能线粒体功能氧化应激是一种氧化应激.

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