相关实验视频
Updated: May 31, 2025
07:30
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
Published on: June 8, 2020
11.9K
通过可视化技术和工具探索RNA-Seq数据分析:对临床应用的机会和局限性的系统审查
Farhana Manzoor1, Cyruss A Tsurgeon2, Vibhuti Gupta1
1Department of Computer Science and Data Science, School of Applied Computational Sciences, Meharry Medical College, Nashville, TN 37208, USA.
Bioengineering (Basel, Switzerland)
|January 24, 2025
概括
本综述调查了用于临床应用的RNA测序 (RNA-seq) 可视化工具. 它强调单细胞RNA-seq是最可视化的数据类型,有助于疾病诊断和预后.
科学领域:
- 基因组学和生物信息学
- 生物医学数据可视化
- 临床转录组学 临床转录组学
背景情况:
- 在临床研究中,RNA测序 (RNA-seq) 对于基因表达分析至关重要.
- 需要有效的可视化工具来解释复杂的RNA-seq数据以获得临床见解.
- 了解基因表达模式有助于诊断和预测疾病的结果.
研究的目的:
- 审查当前的数据可视化技术和RNA测序 (RNA-seq) 分析在临床环境中的工具.
- 评估现有的可视化方法的好处,应用和限制.
- 为研究人员和临床医生提供利用RNA-seq数据的资源.
主要方法:
- 2017年至2024年间发表的研究的系统文献综述.
- 在PubMed,Scopus,Web of Science和IEEE Xplore数据库中进行的搜索.
- 从最初的126项确定中,包括了33项符合PRISMA指南的研究.
主要成果:
- 单细胞RNA-seq数据可视化是最常见的 (56%的研究).
- 还分析了大量RNA-seq数据 (23%) 和循环RNA-seq数据 (18%).
- 长时间的非编码RNA-seq数据可视化是最不常见的 (3%的研究).
结论:
- 有各种可视化工具可用于RNA-seq数据分析.
- 单细胞RNA-seq可视化是临床应用的一个关键领域.
- 本综述提供了一个全面的概述,以指导在临床研究中使用RNA-seq可视化.
相关概念视频
03:21RNA-seq
9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
02:54Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K