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扭曲诱导的上皮转变为半机体转变会产生特定的代谢和线粒体变化

Haleigh N Parker¹, Kayla L Haberman¹, Tolulope Ojo¹

¹Department of Biology, Baylor University, Waco, TX 76798, USA.

|January 24, 2025

概括

皮质到介质酶过渡 (EMT) 通过改变新陈代谢和线粒体结构来促进细胞迁移和生存. 与EMT相关的变化包括葡萄糖分解的增加和线粒体大小的减少,但具体的线粒体动态因细胞类型而异.

科学领域:

细胞生物学细胞生物学
癌症研究癌症研究
代谢工程是代谢工程.

背景情况:

皮质转介质过渡 (EMT) 对于发育,伤口愈合和癌症转移至关重要.
EMT赋予了诸如增强迁移,入侵和抵抗细胞死亡等特征.
了解EMT驱动的代谢和线粒体适应是疾病治疗向的关键.

研究的目的:

在接受EMT的细胞中比较代谢途径和线粒体形态.
在非转化 (HMLE) 和乳腺癌 (MDA-MB-231,MCF7) 细胞系中研究Twist诱导的EMT.
阐明EMT相关的表型变化的上下文性质.

主要方法:

分析不同细胞系中的氧化和糖解代谢.
评估线粒体形态,大小,延长和空间动态.
在HMLE细胞中使用Twist诱导EMT,并与现有的EMT阳性 (MDA-MB-231) 和EMT阴性 (MCF7) 细胞系进行比较.

主要成果:

观察到一种与EMT相关的一般甘油性代谢表型,增加了ATP的产生.
阳性EMT细胞表现出线粒体大小的减少.
线粒体延长和空间动力学有所不同:HMLE Twist细胞具有圆形,分散的线粒体,而MDA-MB-231细胞与MCF7细胞相比具有延长,聚集的线粒体.

结论:

EMT与向糖溶解和改变线粒体形态的转变有关.
线粒体尺寸的缩小是EMT阳性细胞的共同特征.
EMT对线粒体动态的具体影响取决于环境,并且在细胞类型之间有所不同.

关键词:

在EMT中,EMT是EMT.美国TNBC电视台代谢代谢代谢代谢线粒体中的线粒体.

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