外囊子单元EqSec5和EqSec6促进粉状菌的生长和致病性
Jinyao Yin1,2, Xuehuan Zhu1,2, Yalong Chen1,2
1Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Haikou 570228, China.
Journal of fungi (Basel, Switzerland)
|January 24, 2025
概括
外囊复合体 (EqSec5和EqSec6) 对于粉菌的生长和感染至关重要. 沉默这些基因会损害效应体分泌和宿主入侵.
科学领域:
- 分子和细胞生物学分子和细胞生物学
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
背景情况:
- 外囊复合体调节了在真核细胞中囊泡运输和外细胞形成的过程.
- 它在线状真菌中对细胞生长和致病性起着作用.
- 在有义务的生物营养粉菌中,外囊的功能在很大程度上仍然没有被描述.
研究的目的:
- 为了研究外囊复合体在粉状菌中的作用 *Erysiphe quercicola*.
- 识别和描述涉及真菌生长和致病性的外囊子单元.
主要方法:
- 使用GFP融合蛋白识别和定位外囊子单元 (EqSec5,EqSec6).
- 对*EqSec5*和*EqSec6*进行基因沉默,以评估表型效应.
- 酵母双杂交和基因过度表达试验用于研究蛋白质相互作用.
主要成果:
- 在E. quercicola*和Magnaporthe oryzae*中,EqSec5和EqSec6局部存在于尖区域.
- 对*EqSec5*或*EqSec6*的基因沉默导致了显著的生长和感染缺陷.
- 沉默抑制了效应蛋白分泌,破坏了酸生物合成,并触发了宿主防御反应.
结论:
- 外囊复合体对于*E. quercicola*的效应体分泌,细胞生长和宿主感染至关重要.
- 在病变发生过程中,EqSec5和EqSec6相互作用并互补彼此的功能.
- 这项研究阐明了外囊在粉状真菌发育和致病性方面的关键作用.
更多相关视频
11:42Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Published on: September 30, 2016
14.4K
09:03Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
Published on: July 19, 2021
4.9K
相关概念视频
00:50Exocytosis
6.5K
Exocytosis is a process that releases molecules outside the cell. Like other bulk transport mechanisms, exocytosis requires energy.
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
6.5K
01:02Role of Microtubules in Cell Wall Deposition
2.3K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.3K
01:33Overview of Secretory Vesicles
8.4K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.4K