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对L-亚斯巴拉金酶的上游和下游加工进行审查

Kamran Hosseini¹, Tayebeh Zivari-Ghader², Azita Dilmaghani^3,4

¹Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Preparative biochemistry & biotechnology

|January 24, 2025

概括

对于治疗急性淋巴细胞白血病 (ALL) 至关重要的L-asparaginase是由细菌通过发酵产生的. 本综述详细介绍了L-阿斯巴拉金酶生产和净化方法,用于其抗癌应用.

科学领域:

生物化学生物化学
酶学是一种酶学.
生物技术是生物技术.

背景情况:

对于治疗急性淋巴细胞白血病 (ALL) 至关重要,满足全球显著需求.
这种酶消耗L-阿斯巴拉金,这对ALL细胞增殖至关重要.
细菌生产的L-氨酸酶,无论是非重组的还是重组的,都使用了发酵技术,如固态发酵 (SSF) 和浸泡发酵 (SmF).

研究的目的:

审查L-asparaginase生产的上游流程.
用CIPP (捕获,中间净化和抛光) 模型来总结L-阿斯巴拉金酶的下游净化策略.
讨论在L-asparaginase净化中应用的各种染色学和非染色学方法.

主要方法:

对L-酸酶生产的上游发酵过程的审查.
在CIPP框架内分析下游净化技术.
对色谱和非色谱净化方法的讨论.

主要成果:

亚斯巴拉金酶II型表现出优越的特异性活性和抗瘤作用.
有效的净化策略取决于L-asparaginase是否在细胞内或细胞外产生.
在每一个CIPP阶段,用于材料的去除,提取,缩和净化,采用了各种方法.

结论:

对L-阿斯巴拉金酶的产生和净化进行全面的了解对于其治疗用途至关重要.
该CIPP战略提供了一个结构化的方法来净化L-asparaginase.
优化的生产和净化方法提高了L-阿斯巴拉金酶作为抗癌药物的有效性.

关键词:

生物工艺生物工艺在L-asparaginase的使用中.下游加工是下游加工.在生产生产生产生产生产生产.净化净化净化净化在上游加工过程中.

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