囊胚ST1:刺激性肠综合征 (IBS) 患者的蛋白质概况和特定血清免疫球蛋白
Buthaina Darwish1, Ghalia Aboualchamat1, Samar Al Nahhas2
1Department of Animal Biology, Faculty of Science, Damascus University, Damascus, Syria.
在60-95kDa之间的母细胞囊蛋白在刺激性肠综合征 (IBS) 患者中很重要. 这些蛋白质可以作为IBS和其他胃肠道疾病的差异诊断的生物标志物.
科学领域:
- 医学寄生虫学 医学寄生虫学
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
背景情况:
- 胚胎囊 sp. 的情况. 是一个常见的人类肠道寄生虫.
- 卵巢囊感染越来越多地与胃肠道疾病有关,包括刺激性肠综合征 (IBS).
- 区分症状性IBS和非IBS胃肠道疾病在细胞囊阳性个体可能是具有挑战性的.
研究的目的:
- 为了分析Blastocystis ST1 (叙利亚菌株) 的蛋白质概况.
- 为了研究IBS和非IBS患者之间IgG血清免疫球蛋白反应的差异,感染了胚胎细胞.
- 为了确定差异诊断的潜在的血清生物标志物.
主要方法:
- 使用SDS-PAGE.使用Blastocystis ST1溶解物的蛋白质概况.
- 使用IBS和非IBS患者血清进行免疫血清测定.
- 与已知的胚胎囊蛋白蛋白点蛋白质相比,蛋白质反应性的比较.
主要成果:
- 胚囊体ST1溶解体表现出24个蛋白质波段,范围从10到130kDa.
- 蛋白质在27-29,32,39-42和50-51kDa时,在IBS和非IBS患者中显示出类似的免疫性.
- 60-95kDa范围内的蛋白质与IBS患者的血清进行了特定的相互作用.
结论:
- 囊胚蛋白质的60-95kDa,可能是金属蛋白质酶,与IBS有显著的关联.
- 这些特定的细胞囊抗原可以作为血清诊断测试的宝贵工具.
- 鉴定出来的蛋白质可能是IBS在胚胎囊感染个体的差异诊断的潜在生物标志物.
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