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在Chromeno-Pyrroloquinoline探针中使用替代剂驱动的ESIPT用于区分正常和癌细胞

Savita Choudhary¹, Paulkar Suraj², Adarash Kumar Shukla¹

¹Department of Chemistry, Birla Institute of Technology and Science-Pilani (Hyderabad Campus), Hyderabad, 500078, India.

Chemistry, an Asian journal

|January 24, 2025

概括

新的库马林-英多尔光体提供了简单的合成和独特的光物理特性. 一个探测器,ICNMe2,显示聚合诱导的发射和对极性和粘度的高灵敏度,使得癌细胞的检测.

科学领域:

有机化学有机化学
材料科学材料科学材料科学
生物物理化学生物物理化学

背景情况:

光分子传感器通常涉及复杂的合成和有限的功能.
开发多功能且易于合成的光体对于先进的传感应用至关重要.

研究的目的:

基于化库马林-英多尔支架的新型光体的合成和特征.
为了研究这些光体的光物理性质,以应对聚合,溶剂极性和粘度.
评估这些探针用于细胞成像和区分正常细胞和癌细胞的潜力.

主要方法:

基于库马林-英多尔的四种化物的一步合成 (ICH,ICEst,ICOMe,ICNMe2).
光物理性质的表征,包括聚合引起的发射 (AIE) 和聚合引起的火 (ACQ).
使用弗斯特-霍夫曼方程和用于细胞成像的共聚焦显微镜评估粘度和极性灵敏度.

主要成果:

ICNMe2显示聚合诱导的排放 (AIE),而其他探头显示聚合引起的火 (ACQ).
ICNMe2表现出对极性和极性独立粘度的高灵敏度,具有很大的斯托克斯转移 (~175 nm).
通过对焦显微镜的增强发射,ICNMe2成功地区分了正常 (RAW 264.7) 和癌细胞 (A549).

结论:

化库马林-英多尔光体很容易合成,并且具有可调节的光物理性质.
ICNMe2探针显示出作为癌细胞检测敏感和选择性成像剂的前景.
这些发现凸显了这种支架在开发用于生物应用的先进光探头方面的潜力.

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