快速和环保的LC-MS/MS用于兽医学中氨基酸的同时分析
HyunYoung Chae1,2, Jae Won Byun1, Bok-Kyung Ku1
1Animal Disease Diagnosis Division, Animal and Plant Quarantine Agency (APQA), Ministry of Agriculture, Food and Rural Affairs, Gimcheon-si, Republic of Korea.
Veterinary medicine and science
|January 24, 2025
概括
一种新的液态色谱与并联质谱法 (LC-MS/MS) 方法有效地分析了兽医补充剂中的八种必需氨基酸. 这种经过验证的方法确保了产品质量,并提高了动物健康和生产力.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 分析化学 分析化学
- 动物营养 动物营养
背景情况:
- 氨基酸补充剂对于动物健康和生产力至关重要.
- 传统的分析方法复杂,耗时,使用有毒试剂.
- 在兽医中需要有效和可靠的分析方法.
研究的目的:
- 开发和验证一种有效的方法,用于同时分析兽医产品中的八种关键氨基酸.
- 目标氨基酸包括氨酸,氨酸,谷氨酸,氨酸,甲氨酸,甲氨酸,甲氨酸,氨酸和氨酸.
- 为了解决传统分析技术的局限性.
主要方法:
- 使用双重质谱 (LC-MS/MS) 的液体染色学.
- 通过将兽用氨基酸制剂稀释到1mg/L,用50%的乙尼 (ACN) 制备样本,并过 (0.2μm RC过器).
- 在100个注册产品中,对35个选定的产品进行了分析.
主要成果:
- 该方法在0-10mg/L范围内的所有分析物质中显示出出色的线性 (r2 > 0.99).
- 检测极限 (LOD) 在0.04-0.83 mg/L之间,量化极限 (LOQ) 在0.12-2.52 mg/L之间.
- 平均回收率为92.96%-105.61%,相对标准偏差 (RSD) 为0.27%-3.50%,符合监管标准;17.14%的产品未达到合规.
结论:
- 开发的LC-MS/MS方法为兽医同时进行氨基酸分析提供了一种高效可靠的方法.
- 这种方法的实施可以加强氨基酸补充剂的质量控制,改善动物健康.
- 该研究强调了在兽医补充剂行业需要严格的质量管理和持续监测.
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