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Updated: May 31, 2025

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
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低温EM结构和人类NAD激酶的调节

Prakash P Praharaj1, Yang Li2, Charline Mary3

  • 1Children's Medical Center Research Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.

Science advances
|January 24, 2025
PubMed
概括

癌细胞需要增加的尼古丁胺胺氨基二核酸 (NADPH) 才能生长. 研究人员阐明了人类NAD激酶 (NADK) 的结构和调节,揭示了其N-和C-终端尾巴如何控制酶活性和NADPH生产.

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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