细胞外和细胞内抗微生物耐药性之间的不同命运在全面激活污泥和膜生物反应器过程中发生
Mardalisa1, Rongxuan Wang2, Muhammad Adnan Sabar3
1Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan; Department of Marine Science, Faculty of Fishery and Marine Science, Riau University, Riau 28293, Indonesia.
Water research
|January 24, 2025
概括
污水处理厂将细胞外抗菌素耐药性基因 (eARG) 释放到水中. 这项研究发现,废水中的eARG主要来自工厂内的细胞内ARG,而不仅仅来自污泥.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 污水处理 污水处理 污水处理
背景情况:
- 经过处理的废水污水是水生环境中细胞外抗菌素耐药性基因 (eARG) 的重要来源.
- 了解eARG的命运和起源对于减轻它们的传播至关重要.
研究的目的:
- 调查eARG的转化和来源,从废水流入到经过处理的废水.
- 为了比较eARG和细胞内ARG (iARG) 在常规活性污泥 (CAS) 和膜生物反应器 (MBR) 处理过程中的行为.
主要方法:
- 使用枪支元基因组测序来分析eARG和iARG的组成.
- 采集的样本来自一个全尺寸的WWTP的流入,活性污泥和处理的废水.
- 评估了两个处理过程,CAS和MBR.
主要成果:
- eARG与iARG的比例从流入到流出增加,在CAS和MBR中达到近50%.
- eARG的组成从临床上重要的基因转移到污泥和废水中的氨基糖化物抗性基因 (aadA,APH变体).
- 废水eARG的主要来源被确定为废水中的细胞内ARG,而不是从污泥中转移.
结论:
- 在废水处理过程中,不同的机制决定了eARG和iARG的命运.
- 与CAS相比,MBR过程显示eARG的减少更大.
- 废水eARG在很大程度上来源于最终废水中存在的细胞内ARG,这突显了细胞内ARG减少的重要性.
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