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在体外成熟过程中补充N-Acetyl-L-cysteine可以通过调节氧化应激来改善山羊卵细胞发育能力

Yanfei Wang¹, Qingwei Wang¹, Quan Ji¹

¹Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, 550025, China; Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guiyang, 550025, China; College of Animal Science, Guizhou University, Guiyang, 550025, China.

|January 24, 2025

概括

补充N-Acetyl-L-cysteine (NAC) 通过减少氧化应激和增强抗氧化能力来改善山羊卵细胞成熟. 这导致了更好的胚胎发育和整体卵细胞质量.

科学领域:

生殖生物学生殖生物学
氧化压力研究研究氧化压力研究
卵细胞成熟的分子机制

背景情况:

卵细胞质量对于成功的哺乳动物繁殖至关重要,影响受精,胚胎发育和怀孕结果.
由活性氧物种 (ROS) 引起的氧化应激是阻碍体外卵细胞成熟的一个主要因素.
N-乙-L-氨酸 (NAC) 是一种已知的抗氧化剂,可补充谷氨 (GSH) 水平,减轻氧化损伤.

研究的目的:

研究NAC补充剂对山羊卵细胞体外成熟的作用.
通过转录组测序,探索NAC对卵细胞成熟的影响背后的分子机制.
评估NAC对卵细胞发育潜力和胚胎发育的影响.

主要方法:

羊卵细胞在体外成熟,含有不同度的NAC.
评估了卵细胞成熟率,累积细胞扩张和随后的胚胎发育.
测量了反应性氧物种 (ROS) 水平,谷氨 (GSH) 水平和细胞代谢活性.
对MII卵细胞进行了转录组测序,以分析基因表达变化.

主要成果:

1.5毫米的NAC显著增强了卵细胞成熟和累积细胞扩张率.
补充NAC改善了伴生遗传激活胚胎的胚囊率.
NAC降低了ROS积累,增加了GSH水平,并提高了抗氧化能力和代谢活性.
转录组分析显示,NAC调节的基因参与了抗氧化剂防御,能量代谢,亡和炎症.

结论:

补充NAC可以有效地减轻山羊卵细胞成熟过程中的氧化压力.
通过增强抗氧化能力,代谢活性和发育潜力,NAC可以改善卵细胞质量.
该研究阐明了通过NAC积极影响卵细胞成熟和胚胎发育的分子途径.

关键词:

一只山羊是一只山羊.这是一种N-乙-L-氨酸.卵子细胞是卵子细胞.氧化应激是一种氧化应激.一个SIRT1系统.

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