通过连锁生物催化剂的化物生产,使用热稳定性增强的UDP-glycosyltransferase
Guosi Li1, Shanyong Yi1, Haijiao Wang2
1Traditional Chinese Medicine Institute of Anhui Dabie Mountain, Generic Technology Research Center for Anhui Traditional Chinese Medicine Industry, Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, West Anhui University, Lu'an 237012, Anhui, China.
International journal of biological macromolecules
|January 24, 2025
概括
研究人员设计了一种由六种酶组成的生物催化级联,以从L-Tyrosine中有效生产salidroside. 酶工程提高了热稳定性,大大提高了沙利多的产量,并为植物提取提供了可持续的替代品.
科学领域:
- 生物技术是生物技术.
- 酵素工程是什么意思 酵素工程
- 合成生物学 合成生物学
背景情况:
- 沙利德化物是一种有价值的基化物糖化物,来源于rhodiola植物,其生长缓慢,分布有限.
- 目前的生产方法面临的挑战是由于自然来源的局限性和低效的生物合成途径.
研究的目的:
- 开发一种高效和可持续的生物催化级联,用于使用L-Tyrosine作为起始材料生产salidroside.
- 为了克服级联中的瓶,特别是关键的UDP-glycosyltransferase酶的低温稳定性.
主要方法:
- 设计了一种新的六酶生物催化级联.
- 采用计算策略来设计Bacillus licheniformis UDP-glycosyltransferase (BlYjiC M6),提高其热稳定性,以创建突变TSM6.
- 将工程酶集成到级联中,并优化了全细胞生物催化过程.
主要成果:
- 改造的BLYjiC M6突变体TSM6在40°C时呈现出134倍更长的半衰期和13°C更高的明显化温度 (Tm^app).
- 在5L生物反应器中,改进的级联实现了12.8g·L−1的高沙利德化物标位,生产率为0.53g·L−1·h−1.
- 减少了剩余中间体和显著提高了整体salidroside的生产力.
结论:
- 这项研究提出了一种绿色和高效的生物合成过程,用于生产沙利德化物.
- 酶工程是增强生物催化级联和克服生产局限性的强大工具.
- 这种方法为传统的植物提取提供了一种可持续的替代方案,用于像沙利德化这样的有价值化合物.
相关概念视频
01:22Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
01:25Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
01:30Production of Pharmaceuticals
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...
01:25Biofuels
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...