通过基因分子方法检测真菌病原体,使用向大规模平行测序在甲固定组织上:一项回顾性研究
Alexis Trecourt1, Meja Rabodonirina2, Marie Donzel3
1Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, Service de Pathologie Multi-Site - Site Sud, Lyon, France; Université Claude Bernard Lyon 1, Faculté de Médecine Lyon-Sud Charles Mérieux, UR 3738 - CICLY - Equipe Inflammation et immunité de l'épithélium respiratoire, Lyon, France.
概括
这项研究表明,与桑格测序相比,有针对性的大规模并行测序 (MPS) 在甲固定组织中显著改善了真菌识别. 这种组织分子方法有助于在传统方法失败时准确诊断.
科学领域:
- 医学真菌学 医学真菌学
- 分子诊断学 分子诊断
- 病理学 病理学 病理学
背景情况:
- 真菌感染 (FI) 在病理学中很常见.
- 在甲固定型嵌组织 (FT) 中准确识别真菌是具有挑战性的.
- 目前的方法往往缺乏明确的识别.
研究的目的:
- 评估使用向大规模并行测序 (MPS) 来检测FT中的真菌的组织分子方法.
- 为了比较针对性-MPS与桑格测序用于真菌识别的疗效.
- 评估改善真菌诊断的潜在临床影响.
主要方法:
- 追溯分析49个FT与其他的病理FI诊断.
- 菌根模式和组织损伤评估的组织病理学.
- 泛真菌PCR采用ITS-3/ITS-4和MITS-2A/MITS-2B原料,其次是桑格和向的MPS.
主要成果:
- 向MPS在71.4%的样本中成功识别了真菌,明显高于桑格测序 (34.7%).
- 这两种原料套件都显示了针对MPS的高识别率.
- 这种方法可以优化45.8%的患者的护理.
结论:
- 使用向MPS的综合组织分子诊断增强了FT中的真菌鉴定.
- 这种方法在缺乏真菌学识别的情况下提供了关键的诊断信息.
- 改进的诊断有助于选择适当的抗真菌疗法.
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