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优化UroVysion光 In-Situ 杂交反射测试在非典型的尿细胞诊断:一个全面的细胞形态学分析
Moe Thuzar1, Ashley Flowers1, Hidehiro Takei2,3
1Department of Pathology and Translational Pathobiology, Louisiana State University Health Sciences Center, Shreveport, LA, USA.
Annals of clinical and laboratory science
|January 24, 2025
概括
识别特定的细胞学特征可以帮助预测非典型尿细胞 (AUC) 中的恶性瘤. 五个关键参数,包括核扩大和分裂,与尿路癌的UroVysion光现场杂交 (U-FISH) 结果有很强的相关性.
科学领域:
- 尿路病理学 尿路病理学
- 细胞病理学 细胞病理学
- 分子诊断学 分子诊断学
背景情况:
- 尿细胞学对于检测尿癌至关重要.
- 巴黎泌尿细胞学报告系统 (TPS) 标准化了报告,但与非典型尿细胞 (AUC) 斗争.
- 在AUC诊断的变化需要改进客观标准.
研究的目的:
- 为了研究AUCs的细胞形态特征与UroVysion光现场杂交 (U-FISH) 结果之间的相关性.
- 确定在尿路细胞学中预测恶性瘤的特定细胞学参数.
- 评估这些参数在没有反射U-FISH测试的情况下分类病例的潜力.
主要方法:
- 对121例"非典型"诊断的尿细胞学病例进行了回顾性分析.
- 评估了19个细胞形态参数及其与U-FISH结果的关联.
- 与组织学诊断,临床发现和患者病史的相关性.
主要成果:
- 31.4%的病例是U-FISH阳性,U-FISH显示93.5%的敏感性和91.1%的特异性.
- 高非典型的细胞性,核/细胞质比≥0.7,核扩大,多核和线粒分裂与U-FISH阳性显著相关.
- 在TPS AUC标准和U-FISH阳性之间没有发现任何关联;然而,参数组合显示出高的积极预测值 (PPV).
结论:
- 五个特定细胞学参数的组合,或任何四个包括线粒分裂,在恶性瘤中实现了100%的PPV.
- 某些参数组合表明PPV中度,这表明反射U-FISH测试的作用.
- 这些细胞形态特征为改善AUC分类和管理决策提供了潜力.
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