在乙氨基压力下的陆地植物中依赖光的Br-org生产和由光系统介导的化机制
Min Dong1, Kai Zheng1, Zhonglan Shen2
1School of Environmental Science and Engineering, Shandong Key Laboratory of Environmental Processes and Health, Shandong University, Qingdao 266237, China.
暴露于光线会增加在用乙氨基治疗的生菜中形成有毒的化有机化合物. 这通过在质体中生成的活性物种发生,突出显示了一种新的生物光化学化途径.
科学领域:
- 环境化学环境化学
- 植物科学 植物科学
- 毒理学 毒理学 毒理学
背景情况:
- 化有机污染物 (Br-org) 由于内分泌,神经毒素和生殖作用而带来风险.
- 关于植物中类污染物的化过程以及相关的食品风险,存在知识缺口.
研究的目的:
- 为了研究在乙氨基 (ACE) 压力下,在生菜中因光引起的化有机化合物的生成.
- 为了阐明植物叶绿体内的生物光化学化机制.
主要方法:
- 里埃变换红外光谱 (FTIR) 检测C-Br键.
- 高性能液体染色学-并联质谱学 (HPLC-MS/MS) 用于识别转化产品.
- 对颜料含量,过氧化 (H2O2) 和单一氧气 (1O2) 含量进行分析.
主要成果:
- 与黑暗条件相比,光线显著增加了ACE压力中的有毒Br-org积累.
- 离子 (Br-) 在叶绿体内被氧化为活性物种 (RBS),由活性氧物种 (ROS) 和三重叶绿素 (3Chl*) 促进.
- 确定了六种转化产物,涉及甲基化,氧化和氧化,随后是化通路.
结论:
- Br-可以进入叶绿体,并在ACE压力下改善它们的结构,这是一个新的发现.
- 在质体中阐明了一种生物光化学化机制,与酶催化路径不同.
- 这项研究为评估和预防食品中新出现的污染物的风险提供了关键的见解.
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