承载力的细胞粘附环介导了与表面结合的颗粒的细胞分裂
Subhankar Kundu1, Kaushik Pal2, Arghajit Pyne1
1Hoxworth Center, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.
Nature communications
|January 24, 2025
概括
巨细胞使用独特的细胞粘附环 (PAR) 来分离附着的颗粒. 这些β2整蛋白结构约束和断颗粒,这对于跨物种高效的细胞化至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 生物物理学的生物物理.
背景情况:
- 微粒子,包括病原体,强烈粘附于宿主细胞.
- 巨细胞在细胞分裂过程中将这些与表面结合的颗粒分离出来的机制尚不清楚.
研究的目的:
- 阐明了细胞分裂过程中巨细胞颗粒脱离的机制.
- 为了识别细胞结构和分子参与者参与分离与表面结合的粒子.
主要方法:
- 对与微粒子相互作用的巨细胞的活细胞成像.
- 免疫光显微镜可用于可视化actin和integrin动态.
- 功能性测试,以评估粘附结构在粒子内部化中的作用.
主要成果:
- 巨细胞形成独特的β2整合素介导的粘附结构,称为细胞粘附环 (PAR),围绕着表面结合的粒子.
- PARs支持环状的动因收缩,将颗粒从基质中挤出.
- PARs中的整体素维持张力,这对于表面结合颗粒的高效脱离和细胞化至关重要.
- 在不同的巨细胞类型 (老鼠,人类,鱼类) 和粒子类型 (微粒,大肠杆菌) 中,PAR形成是保留的.
结论:
- 细胞粘附环 (PARs) 代表了巨细胞脱离和内化表面粘附颗粒的新机制.
- PARs利用β2整合素介导的粘附和actin动力学来克服颗粒对基板的粘附.
- 这种保存的机制突显了巨细胞在吞具有挑战性的目标时的适应能力.
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