通过综合生理学和转录基因分析,揭示了Acer fabri在适应低温压力的分子机制
Gongwei Chen1,2, Dandan Zhang2, Fengyuan Chen2
1School of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, No. 19 Wenchang East Road, Jurong, 212400, China.
Scientific reports
|January 24, 2025
概括
Acer fabri通过增加素,可溶性糖和可溶性蛋白质水平来应对低温. 确定了关键的生理指标和基因表达模式,包括转录因子和MAPK通路,以了解冷压分子机制.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 树是一种有价值的装饰树种.
- 低温限制了它的生长和分布.
- 在A. fabri中,冷应激反应的分子机制尚不清楚.
研究的目的:
- 为了阐明Acer fabri对低温压力的反应背后的分子机制.
- 确定与寒冷适应有关的关键生理和遗传因素.
主要方法:
- 对普罗林,可溶糖,可溶蛋白,过氧化酶 (POD) 和超氧化物脱酶 (SOD) 的生理分析.
- 转录组测序以识别差异表达基因 (DEGs).
- 正交部分最小平方差异分析 (OPLS-DA) 和权重基因同表达网络分析 (WGCNA).
主要成果:
- 在寒冷压力下增加了proline,可溶糖和可溶蛋白质含量.
- SOD被确定为一个关键的生理指标;POD和SOD活动显示动态变化.
- 转录组分析显示了832个转录因子和在代谢,植物激素和MAPK信号通路中的丰富.
- WGCNA确定Af0048792和Af0026061是潜在的重要基因,可能涉及NAC和MIKC转录因子的相互作用.
结论:
- 这项研究提供了Acer fabri对低温压力的生理和分子反应的见解.
- 确定了关键的基因和通路,为改善A. fabri.寒冷耐受性提供了目标.
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