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使用调制光学计算相位显微镜对细胞和纳米粒子进行成像

Xuan Liu¹, Rupak Bhakta², Emily Kryvorutsky³

¹Department of Electrical and Computer Engineering, New Jersey Institute of Technology, Newark, NJ, 07102, USA. xliu@njit.edu.

Scientific reports

|January 24, 2025

概括

我们开发了模块化光学计算相位显微镜 (M-OCPM) 进行细胞-纳米粒子相互作用的无标签成像. 这项技术克服了灵敏度-分辨率的权衡,使得纳米粒子动态在药物输送系统的详细研究.

科学领域:

生物物理学的生物物理.
纳米技术纳米技术
光学成像技术的成像

背景情况:

纳米粒子 (NP) 对药物输送至关重要,需要了解细胞-NP相互作用.
定量相位成像为这些动态提供了无标签的洞察力.
现有的相位成像方法面临着灵敏度-分辨率的权衡.

研究的目的:

开发一种新的相位成像技术,克服灵敏度-分辨率的权衡.
为了使细胞-纳米粒子相互作用的高灵敏度,高分辨率,无标签成像.
评估用于研究生物系统中纳米粒子动态的新技术的可行性.

主要方法:

开发了模块化光学计算相位显微镜 (M-OCPM),使用低相干干扰度和光学计算.
M-OCPM使用光学计算用于里埃变换和时间调制,以规避灵敏度-分辨率权衡.
用各种样本评估M-OCPM性能,包括培养细胞和纳米颗粒.

主要成果:

M-OCPM证明了无标签成像,具有纳米尺度位移灵敏度和~250nm分辨率.
成功成像了与培养细胞相互作用的纳米粒子.
附着纳米颗粒与介质中的纳米颗粒的不同信号特征.

结论:

M-OCPM有效地克服了相位成像中的灵敏度-分辨率权衡.
该技术可用于研究复杂的细胞-纳米粒子相互作用.
为优化基于纳米粒子的药物输送系统提供了强大的工具.

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