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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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相关实验视频

Updated: May 31, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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使用调制光学计算相位显微镜对细胞和纳米粒子进行成像.

Xuan Liu1, Rupak Bhakta2, Emily Kryvorutsky3

  • 1Department of Electrical and Computer Engineering, New Jersey Institute of Technology, Newark, NJ, 07102, USA. xliu@njit.edu.

Scientific reports
|January 24, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了模块化光学计算相位显微镜 (M-OCPM) 进行细胞-纳米粒子相互作用的无标签成像. 这项技术克服了灵敏度-分辨率的权衡,使得纳米粒子动态在药物输送系统的详细研究.

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科学领域:

  • 生物物理学的生物物理.
  • 纳米技术 纳米技术
  • 光学成像技术的成像

背景情况:

  • 纳米粒子 (NP) 对药物输送至关重要,需要了解细胞-NP相互作用.
  • 定量相位成像为这些动态提供了无标签的洞察力.
  • 现有的相位成像方法面临着灵敏度-分辨率的权衡.

研究的目的:

  • 开发一种新的相位成像技术,克服灵敏度-分辨率的权衡.
  • 为了使细胞-纳米粒子相互作用的高灵敏度,高分辨率,无标签成像.
  • 评估用于研究生物系统中纳米粒子动态的新技术的可行性.

主要方法:

  • 开发了模块化光学计算相位显微镜 (M-OCPM),使用低相干干扰度和光学计算.
  • M-OCPM使用光学计算用于里埃变换和时间调制,以规避灵敏度-分辨率权衡.
  • 用各种样本评估M-OCPM性能,包括培养细胞和纳米颗粒.

主要成果:

  • M-OCPM证明了无标签成像,具有纳米尺度位移灵敏度和~250nm分辨率.
  • 成功成像了与培养细胞相互作用的纳米粒子.
  • 附着纳米颗粒与介质中的纳米颗粒的不同信号特征.

结论:

  • M-OCPM有效地克服了相位成像中的灵敏度-分辨率权衡.
  • 该技术可用于研究复杂的细胞-纳米粒子相互作用.
  • 为优化基于纳米粒子的药物输送系统提供了强大的工具.