相关实验视频

Updated: May 31, 2025

Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury

Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury

Published on: February 26, 2017

在辐射引起的肺损伤的发展中cGAS-STING通路的作用

Xinyao Zhao¹, Lehui Du², Na Ma²

¹Department of Radiation Oncology, Shijingshan Teaching Hospital of Capital Medical University, Shijingshan Hospital, Beijing, China.

Journal of cancer research and clinical oncology

|January 24, 2025

概括

循环GMP-AMP合成酶 (cGAS) 刺激干扰素基因 (STING) 途径被辐射诱导肺损伤 (RILI) 激活. 抑制这种途径可能会减少放射治疗后的肺炎和纤维化.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
放射疗法是一种放射治疗.

背景情况:

辐射诱导肺损伤 (RILI) 是一个重要的临床挑战,限制胸部放射治疗的疗效.
驱动RILI致病的精确机制仍然不完全理解.
参与细胞质DNA感应和炎症的cGAS-STING通路是一个潜在的关键参与者.

研究的目的:

阐明cGAS-STING途径在RILI发展中的作用.
调查调节cGAS-STING通路是否可以减轻RILI.

主要方法:

通过全胸部辐射建立了一个临床前小鼠RILI模型.
利用STING激动剂 (DMXAA) 和抗剂 (C-176) 在体内调节cGAS-STING通路.
采用西方抹黑和ELISA用于蛋白质表达分析和组织染色以评估组织.

主要成果:

辐射导致dSDNA积累,并激活肺组织中的cGAS-STING通路.
使用C-176减轻了辐射诱导的肺炎和纤维化.
实验室研究证实,辐射诱导的dsDNA释放激活了cGAS-STING,促进了M1巨细胞的两极分化和促炎性细胞因子的产生.

结论:

这项研究表明,cGAS-STING途径与RILI发展之间存在显著的关联.
针对cGAS-STING通路提供了一个潜在的治疗策略,用于在临床环境中管理RILI.

关键词:

在M1极化中,M1极化巨细胞是一个巨细胞.辐射引起的肺损伤.cGAS-STING 信号通道的信号通道

更多相关视频

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...