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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: May 31, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

细胞储存条件的影响单细胞蛋白质组景观

Bora Onat^1,2,3, Amanda Momenzadeh^1,2,3, Ali Haghani³

¹Department of Computational Biomedicine, Cedars Sinai Medical Center, Los Angeles, California 90048, United States.

Journal of proteome research

|January 24, 2025

概括

此摘要是机器生成的。

为单细胞蛋白质组学 (SCP) 运输细胞需要了解存储效应. 冷或冷储存会显著改变细胞大小和蛋白质组概况,影响SCP数据质量.

关键词:

在HEK293T的基础上.在 LC-MS 中.这是一个SCP SCP.单细胞蛋白质组学单细胞蛋白质组学储存条件储存条件储存条件时间STOF-SCP.

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Published on: May 22, 2019

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Last Updated: May 31, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

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Published on: December 9, 2022

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

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科学领域:

蛋白质组学是指蛋白质组学.
细胞生物学细胞生物学
质谱测量质量谱测量

背景情况:

单细胞转录组学 (SCT) 进行了先进的细胞异质性研究.
单细胞蛋白质组学 (SCP) 提供了一个更具功能性的细胞动态视角.
在SCP的后勤挑战需要远程样本处理,包括运输.

研究的目的:

研究各种细胞储存条件对单细胞蛋白质组分析的影响.
评估运输和储存如何影响蛋白质组合和细胞形态.

主要方法:

使用数据独立获取 (DIA) 与并行积累串行碎片化 (diaPASEF).
对比了293T细胞的蛋白质组概况,这些细胞在37°C (对照细胞),4°C (一夜间) 和196°C (液) 的温度下存储.
分析了细胞直径,延长和蛋白质组合的变化.

主要成果:

冷 (4°C) 和冷 (-196°C) 的储存条件都导致细胞形态 (直径,延长) 的显著改变.
储存在单细胞水平上显著影响了蛋白质组合.
与冷藏和冷储存相比,对照条件 (37°C) 的变化很小.

结论:

细胞储存条件极大地影响细胞形态和蛋白质组形状.
结果为优化SCP样本准备和确保数据完整性提供了必要的技术见解.
这项研究解决了一个关键的技术缺口,用于广泛采用远程SCP分析.