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从单细胞层面的洞察:在矮人冲浪 Mulinia lateralis 中的精子发生过程中的血统轨迹和体质-胚芽相互作用

Yajuan Li^1,2,3, Huilan Wei⁴, Xiaoting Dai²

¹Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China.

|January 25, 2025

概括

这项研究绘制了矮人冲浪丸的单细胞转录组图,揭示了精子发生过程中的关键基因和细胞通信. 这些发现提升了对软体动物生殖细胞发育和潜在操纵技术的理解.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
细胞生物学细胞生物学

背景情况:

精子生成是一个复杂的分化过程,对繁殖至关重要.
了解精子发生的分子驱动因素,特别是无脊椎动物,仍然有限.
矮海 (Mulinia lateralis) 为研究双生殖生物学提供了一个有价值的模型.

研究的目的:

通过单细胞RNA测序,全面描述Mulinia lateralis中的丸细胞种群.
识别关键的基因和分子通路,控制胚胎细胞的发育和分化.
探索性腺体内的细胞间通信网络.

主要方法:

测序超过4,600个丸细胞的单细胞RNA.
细菌和体细胞种群的识别和表征.
伪时代分析以推断发育轨迹.
权重基因联合表达网络分析 (WGCNA) 以确定关键的转录因子.

主要成果:

描述了四种生殖细胞种群和三种体细胞类型.
揭示了不同的细胞周期状态和不间断的胚胎细胞发育轨迹.
确定了参与精子生成的关键转录因子 (例如,MYC,SREBF1,SOXH) 和信号通路 (FGF,TGF-β).
发现了体和生殖细胞之间广泛的双向通信.

结论:

提供了一个详细的单细胞转录组图的 Mulinia lateralis 性腺.
增强对精子发生过程中生殖细胞命运过渡的理解.
为开发在软体动物中的生殖细胞操纵技术奠定了基础.

关键词:

这种植物是Mulinia lateralis.发生性腺位 (Gonadal niche) 是一个精子发生是精子生成.测试 (Testis) 是一个测试.在WGCNA中,WGCNA是WGCNA.这就是scRNA-seqq.

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