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研究的质量恶化和代谢变化在冷藏储存期间被破坏细菌诱导的研究
Hanzheng Dou1,2,3, Wenxiu Zhu1,2,3, Siyang Chen1,2,3
1School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Foods (Basel, Switzerland)
|January 25, 2025
概括
三种主要的细菌菌株,Psychrobacter immobilis,Shewanella putrefaciens和Photobacterium swingsii,显著影响了的腐烂. 代谢学发现了监测的新鲜度的潜在标志物.
科学领域:
- 海洋微生物学 海洋微生物学
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
背景情况:
- 水产食品的质量,特别是,在冷藏期间由于腐烂细菌而显著降低.
- 主导腐烂细菌在退化的具体作用尚未完全理解.
- 识别这些细菌及其代谢副产品对于质量控制至关重要.
研究的目的:
- 调查肉中三种主要细菌菌株的腐烂能力.
- 用LC-MS非向代谢学来分析用这些细菌接种的肉中的代谢变化.
- 为了确定损坏的潜在生物标志物.
主要方法:
- 用Psychrobacter immobilis,Shewanella putrefaciens和Photobacterium swingsii的纯种植物对肉进行注射.
- 使用生物化学指标评估腐烂:总挥发性基本 (TVB-N),pH,TBARS和体重减轻.
- 通过基于LC-MS的非向代谢学分析,对腐烂的肉进行代谢学分析.
主要成果:
- 赛克罗巴克特不动菌和谢瓦内拉腐烂菌表现出显著的腐烂能力.
- 照片细菌swingsii虽然占主导地位,但其破坏能力较低,但产生微生物脂质酶,有助于脂肪氧化.
- 代谢分析确定了7,8-Dimethoxy-3-(4-methoxyphenyl) -4-oxo-4H-chromen-5-yl-2-O-pentopyranosylhexopyranoside,1,2,3,6-Tetrahydropyridine-4-carboxylic acid,propionic acid和L-phenylalanine作为潜在的破坏标志物,这些标志物可以被认为是潜在的破坏标志物.
结论:
- 心理细菌 (Psychrobacter immobilis) 和雪瓦内拉 (Shewanella putrefaciens) 是导致损坏的关键因素.
- 照片细菌swingsii在脂肪氧化中发挥作用,影响整体质量.
- 鉴定到的代谢物为开发新的鲜度监测技术提供了有希望的目标.
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